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草业学报  2015 

外源钙和赤霉素对干旱胁迫下苜蓿幼苗生理特性的影响

DOI: 10.11686/cyxb2014502, PP. 89-96

Keywords: 苜蓿幼苗,CaCl2,GA3,复合液,干旱胁迫

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Abstract:

以紫花苜蓿幼苗为材料,采用营养钵沙培1/2Hoagland营养液法,研究PEG-6000模拟干旱胁迫下不同浓度CaCl2(0,5,10,20mmol/L)、GA3(0,50,100,150mg/L)及不同比例复合液[CaCl2∶GA3设体积比1∶1(T1∶1)、1∶2(T1∶2)、2∶1(T2∶1)及质量比1∶1(Z1∶1)]处理对苜蓿幼苗生理效应的影响,并用隶属函数法进行综合评价,探讨外源钙(CaCl2)、赤霉素(GA3)及其复合液对干旱胁迫下苜蓿幼苗缓解的生理效应及其适宜浓度、适宜复合液比例。结果表明,1)干旱胁迫下,苜蓿幼苗叶片叶绿素相对含量降低,而相对电导率、MDA含量、Pro含量、SOD活性以及POD活性均显著增加。2)与干旱胁迫下相比,经适宜浓度的CaCl2、GA3及复合液处理后,可缓解幼苗叶绿素的降解,可降低相对电导率,减少MDA的积累,保持较高的SOD、POD活性。3)利用隶属函数法得出:CaCl2处理以10mmol/L效果最好;GA3处理以100mg/L效果最好;CaCl2+GA3复合液处理以T1∶1最好,且优于单独的10mmol/LCaCl2、100mg/LGA3处理。各个处理对苜蓿幼苗干旱胁迫下的缓解效应由强到弱依次为:T1∶1>10mmol/LCaCl2>T2∶1>CK1>T1∶2>100mg/LGA3>Z1∶1>CK2。

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