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草业学报  2015 

外源氮素形态对紫花苜蓿不同生育期根系特性的影响

DOI: 10.11686/cyxb2014269, PP. 53-63

Keywords: 紫花苜蓿,NO3--N,NH4+-N,生育期,根系特性

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Abstract:

在完全营养液的条件下,采用砂培法,研究了3种外源氮素形态配比(NO3--N,NH4+-N以及NO3--N∶NH4+-N为1∶1)和3个氮素水平(0,105,210mg/L)对“甘农3号”紫花苜蓿整个生育期根系特性的影响。结果表明,不同形态氮素处理下紫花苜蓿的根系生物量、根表面积、根体积、根系活力、根瘤数、根瘤重和固氮酶活性均显著高于CK,NO3--N和NH4+-N混合培养下效果最好,NH4+-N培养下次之,NO3--N培养下最低,但对根系平均直径的影响并不大。随着氮素水平的增加,各形态配比下紫花苜蓿的根系生物量、根表面积、根体积、根系活力、根瘤数、根瘤重和固氮酶活性均呈增加的变化趋势,各指标在O3--N+NH4+-N的浓度为210mg/L时,达到最大值。整个生育期,各处理下紫花苜蓿的根系生物量、根表面积、根系活力、根瘤数、根瘤重和固氮酶活性均在苗期、现蕾期、盛花期差异比较明显,结荚期和鼓粒期差异不显著,各指标之间均有不同程度的相关性。

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