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草业学报  2015 

西藏豆科牧草青贮饲料中耐低温优良乳酸菌的筛选

DOI: 10.11686/cyxb2014312, PP. 99-107

Keywords: 豆科牧草,青贮饲料,乳酸菌,分离,鉴定

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Abstract:

本研究对西藏地区豆科牧草青贮饲料中的乳酸菌进行分离、鉴定,以期得到优良的乳酸菌菌株。以苜蓿和箭筈豌豆青贮饲料为试验材料,共分离得到6株同型发酵乳酸菌,经传统微生物培养和16SrRNA序列分析,3株(LCG9,CG35和AG11)为戊糖片球菌,2株(LCG3和LAG1)为植物乳杆菌,1株(LA3)为弯曲乳杆菌;除AG11和LAG1在5℃,LCG9和AG11在pH3.0和8.0生长微弱外,其余乳酸菌菌株均在5~20℃,pH3.0~8.0及3.0%和6.5%NaCl培养液中生长良好。从耐低温特性、产酸能力和发酵速率3个指标考虑,植物乳杆菌LCG3最适宜用于西藏青贮饲料生产的乳酸菌菌种。

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