全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
草业学报  2015 

四个抗草甘膦基因的抗性比较

DOI: 10.11686/cyxb20150519, PP. 159-166

Keywords: 比较,草甘膦,抗性,基因

Full-Text   Cite this paper   Add to My Lib

Abstract:

草甘膦抗性是当代农业重要的农艺性状。为了选择最佳的抗性基因用于植物筛选标记,通过选取cp4-epsps、GR79-epsps、gat4621和GAT四个草甘膦抗性基因进行比较,在拟南芥中验证各基因对草甘膦的抗性。4个基因的转化苗经4个浓度的草甘膦喷施检测,结果显示,GAT类基因的抗性明显高于epsps类基因,基因间的差异达到了极显著水平。gat4621基因对草甘膦的抗性最好,在各浓度下存活率均表现为最高值,且抗性稳定。国内发掘的GAT基因对草甘膦也表现出良好的抗性。GAT类基因的拟南芥转化苗在喷施高浓度草甘膦条件下出现严重抽苔抑制;但在停施草甘膦后抑制解除,且可以开花结实。转epsps类基因可以获得高抗草甘膦植株,但在花期喷施草甘膦将造成败育。从基因大小及抗性强弱考虑,GAT类基因作为筛选标记基因应该更为适合。

References

[1]  Preuss S B, Meister R, Xu Q, et al . Expression of the Arabidopsis thaliana BBX32 gene in soybean increases grain yield. PloS one, 2012, 7(2): 30717.
[2]  Yan X H, Wang H, Ye Y Y, et al . YBA100: An ease-of-use binary vector with LoxP-FRT recombinase site for plant transformation. Molecular Plant Breeding, 2012, 10(3): 371-379.
[3]  Clough S J, Bent A F. Floral dip: a simplified method for Agrobacterium -mediated transformation of Arabidopsis thaliana . The Plant Journal, 1998, 16(6): 735-743.
[4]  Zeng G, Ye Y Y, Yan X H, et al . Quick detection of turnip mosaic virus by one-step multiple RTPCR. Acta Agriculture Boreali-Sinica, 2012, 27(3): 102-107.
[5]  Joshi C P, Zhou H, Huang X, et al . Context sequences of translation initiation codon in plants. Plant Molecular Biology, 1997, 35(6): 993-1001.
[6]  Pline-Srnic W. Technical performance of some commercial glyphosate-resistant crops. Pest Management Science, 2005, 61(3): 225-234.
[7]  Gaines T A, Zhang W, Wang D, et al . Gene amplification confers glyphosate resistance in Amaranthus palmeri . Proceedings of the National Academy of Sciences, 2010, 107(3): 1029-1034.
[8]  王慧, 闫晓红, 徐杰, 等. 我国抗草甘膦基因的发掘现状. 农业生物技术学报, 2014, 22(1): 109-118.
[9]  林敏, 梁爱敏, 陆伟, 等. 高耐受草甘膦的EPSP合酶及其编码序列[P]. 中国专利,ZL200710177090.1, 2010.
[10]  林敏, 顿宝庆, 陆伟, 等. 草甘膦乙酰转移酶基因及其应用[P]. 中国专利,ZL200510086626.X., 2007.
[11]  林敏, 王旭静, 顿宝庆. 培育抗草甘膦植物的双价表达载体[P]. 中国专利,ZL200710118968.4. 2010.
[12]  闫晓红, 王慧, 叶艳英, 等. 一个含LoxP-FRT重组酶位点及易于操作的植物表达双元载体pYBA100. 分子植物育种, 2012, 10(3): 371-379.
[13]  曾钢, 叶艳英, 闫晓红, 等. 简化一步多重RT-PCR法快速检测芜菁花叶病毒. 华北农学报, 2012, 27(3): 102-107.
[14]  Amrhein N, Deus B, Gehrke P, et al . The site of the inhibition of the shikimate pathway by glyphosate: II. Interference of glyphosate with chorismate formation in vivo and in vitro . Plant Physiology, 1980, 66(5): 830-834.
[15]  Gruys K J, Walker M C, Sikorski J A. Substrate synergism and the steady-state kinetic reaction mechanism for EPSP synthase from Escherichia coli . Biochemistry, 1992, 31(24): 5534-5544.
[16]  Wang H, Yan X H, Xu J, et al . Current status of excavation glyphosate-resistant gene in China. Journal of Agricultural Biotechnology, 2014, 22(1): 109-118.
[17]  Padgette S R, Kolacz K H, Delannay X, et al . Development, identification, and characterization of a glyphosate tolerant soybean line. Crop Science, 1995, 35: 1451-1461.
[18]  Forlani G, Mangiagalli A, Nielsen E, et al . Degradation of the phosphonate herbicide glyphosate in soil: evidence for a possible involvement of unculturable microorganisms. Soil Biology and Biochemistry, 1999, 31(7): 991-997.
[19]  Green J M, Hazel C B, Forney D R, et al . New multiple-herbicide crop resistance and formulation technology to augment the utility of glyphosate. Pest Management Science, 2008, 64(4): 332-339.
[20]  Lin M, Liang A M, Lu W, et al . High Glyphosate Tolerant EPSP Synthase and its Coding Sequence[P]. China Patent, ZL200710177090.1, 2010.
[21]  Cao G, Liu Y, Zhang S, et al . A novel 5-enolpyruvylshikimate-3-phosphate synthase shows high glyphosate tolerance in Escherichia coli and tobacco plants. PloS one, 2012, 7(6): e38718.
[22]  Lin M, Dun B Q, Lu W, et al . Glyphosate Acetyltransferase Gene and its Application[P]. China Patent, ZL200510086626.X, 2007.
[23]  Lin M, Wang X J, Dun B Q. A Bivalent Expression Vector for Generating Glyphosate-resistant Plant[P]. China Patent, ZL200710118968.4, 2010.
[24]  Komori T, Imayama T, Kato N, et al . Current status of binary vectors and superbinary vectors. Plant Physiology, 2007, 145(4): 1155-1160.
[25]  Zhou H, Arrowsmith J W, Fromm M E, et al . Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation. Plant Cell Reports, 1995, 15(3-4): 159-163.
[26]  Yao L, Yan X, Wang H, et al . pYBA: A series ease-of-use binary vectors for plant transformation. Plant Genomics in China XIII[C]. 2012: 212.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133