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草业学报  2015 

吸胀冷害下外源NO对紫花苜蓿种子萌发及抗氧化性的影响

DOI: 10.11686/cyxb20150410, PP. 87-94

Keywords: 一氧化氮,紫花苜蓿,吸胀冷害,抗氧化性

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Abstract:

用浓度为0.1mmol/L外源NO供体硝普钠(sodiumnitroprusside,SNP)处理4℃吸胀冷害下紫花苜蓿种子,来探讨吸胀冷害下NO对紫花苜蓿种子萌发及氧化损伤的影响。试验设计4个处理,分别为A:对照组(CK)为蒸馏水;B:4℃吸胀冷害组(Chilling);C:SNP;D:Chilling+SNP,每个处理重复3次。结果表明:1)紫花苜蓿种子经SNP处理12h转入25℃继续培养84h后,Chilling+SNP组比Chilling组发芽率、活力指数、发芽指数提高了14.37%,34.17%,1.29%。2)4℃吸胀冷害12h后,Chilling+SNP组同Chilling组相比超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性分别提高了149.26%,60.35%,过氧化氢(H2O2)含量及超氧阴离子(·O2-)产生速率则降低了10.11%,27.61%。3)SNP对4℃吸胀冷害12,24和48h的种子体内羟自由基(·OH)的清除均呈下降趋势,Chilling+SNP组的清除率均高出SNP组,Chilling+SNP组在冷害12h时清除率达最大值97.5%。综上所述,施加外源NO能促进种子的萌发,提高抗氧化酶活性,降低了活性氧对种子造成的伤害。

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