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草业学报  2015 

NO参与Spd诱导白三叶抗氧化酶活性及其基因表达

DOI: 10.11686/cyxb20150417, PP. 140-147

Keywords: 白三叶,一氧化氮,亚精胺,抗氧化酶,基因表达

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Abstract:

以‘拉丁诺’白三叶为试材,采用药理学实验,探讨一氧化氮(NO)信号在外源亚精胺(Spd)诱导抗氧化酶活性及其基因表达中的作用。研究结果显示,20μmol/L的外源Spd可显著提高白三叶叶片硝酸还原酶(NR)和一氧化氮合酶(NOS)活性,诱导白三叶离体叶片NO积累,并且具有时间效应,在处理第2h达到最大值;50μmol/LNO清除剂牛血红蛋白(Hb)、5mmol/L硝酸还原酶(NR)抑制剂偏钒酸钠(NaVO3)以及200μmol/L一氧化氮合酶(NOS)抑制剂NG-硝基-L-精氨酸甲酯盐酸盐(L-NAME)处理均可不同程度地逆转Spd诱导的NO含量的升高。外源Spd亦可提高白三叶叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)的活性及其基因的相对表达量,而Hb、NaVO3和L-NAME不同程度地抑制了Spd诱导的SOD、POD、CAT、APX酶活性及其基因表达。以上结果表明:Spd可能通过激活硝酸还原酶和一氧化氮合酶途径诱导产生NO,且NO信号参与了Spd调控白三叶叶片抗氧化酶活性及其基因表达。

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