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草业学报  2015 

外源H2O2对沙打旺抗黄矮根腐病的影响

DOI: 10.11686/cyxb20150311, PP. 108-114

Keywords: 沙打旺,埃里砖格孢,H2O2,AsA,生理活性

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Abstract:

本研究以沙打旺抗病和感病品种为材料,接种埃里砖格孢孢子悬浮液后分别施加外源H2O2及其清除剂AsA,通过统计发病率、病情指数及测定POD、PPO、CAT、SOD、Glu和Cht六种病程相关蛋白酶活性,研究H2O2对沙打旺抗黄矮根腐病的影响。结果表明,接种埃里砖格孢后2种沙打旺H2O2含量高于未接种植株,且感病品种在38hH2O2含量最高,为929μmol/g,抗病品种最高值出现在24h,为986μmol/g。抗病、感病沙打旺发病率、病情指数在H2O2处理后最低,施加AsA最高。H2O2处理各种酶活性都有不同程度的增加,且对抗病品种酶活性提高大于感病品种,表明外施H2O2可以减缓沙打旺黄矮根腐病的发生和侵染。表明H2O2与沙打旺抗病性紧密相关,并通过调节病程相关酶活性来增强沙打旺对黄矮根腐病的抗性,减少侵染率。

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