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草业学报  2015 

葡萄抗寒性综合评价方法的建立

DOI: 10.11686/cyxb20150307, PP. 70-79

Keywords: 葡萄,抗寒性,综合评价

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Abstract:

为建立葡萄抗寒性的综合评价方法,以7个葡萄品种的枝条为试材,通过人工模拟低温的方法,分别于10、-5、-15、-25、-35、-45℃下处理葡萄枝条,测定枝条可溶性糖、可溶性蛋白、丙二醛、游离脯氨酸、组织含水量、相对电导率等抗寒相关指标,根据抗寒性指标在低温处理过程中的变化,确定葡萄枝条抗寒性指标的有效低温适用范围。并以相对电导率结合Logistic方程计算葡萄枝条的半致死温度(LT50),以LT50为葡萄抗寒能力的衡量指标,用相关分析法确定评价葡萄抗寒性的5个主要相关指标。用主成分分析法将5个指标转化为综合的主成分,确定指标权重,并结合隶属函数法建立葡萄抗寒的评价方法,运用隶属度函数值求得各葡萄抗寒性的综合评价指数Y。根据综合指数判断抗寒性强弱顺序为:双优>左优红>北冰红>贝达>5BB>5C>黑比诺。结果表明:葡萄枝条可溶性糖含量、可溶性蛋白含量、游离脯氨酸含量、丙二醛含量、相对电导率等与葡萄抗寒性有极显著或显著相关关系,因此,运用主成分分析法结合隶属度函数法,来综合评价葡萄的抗寒性将更加准确、科学。

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