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草业学报  2010 

几种禾本科牧草内生细菌的分布特性

, PP. 124-131

Keywords: 禾本科牧草,内生细菌,脂肪酸检测,分布特性

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Abstract:

不同禾本科牧草植株内生细菌含量差异显著,杂交狼尾草闽牧六号内生细菌含量最高,达26.800×104CFU/g。具体到各个部位,禾本科牧草不同部位内生细菌含量差异也显著。通过微生物鉴定系统对内生菌进行鉴定的结果表明,64株内生细菌中与MIDI数据库比较相似性指数大于0.500,可确定其菌种名称的有50株,属于19个种,隶属于12个属。以分离到的19种内生菌为样本,以各菌株在各部位出现与否为指标,用类平均法对数据进行系统聚类分析,当λ=8.35时,可将19种内生细菌分为4个大类群。从聚类结果显示,内生菌与宿主植株和不同分离部位都存在相关性,与宿主植株的关系更密切。

References

[1]  刘蕴哲, 何劲, 张杰, 等. 植物内生真菌及其活性代谢产物研究进展[J]. 菌物研究, 2005, 3(4): 30-36.
[2]  Vogl A E. Mehl und die anderen Mehlprodukte der Cerealien und Leguminosen. Z.[J]. Nahrungs mittel-Untersuchung, Hyg. Warenkunde, 1898, 12: 25-29.
[3]  何劲, 刘蕴哲, 康冀川. 植物内生菌及其在农业和医学上的用途[J]. 贵州农业科学, 2006, 34(3): 113-115.
[4]  姜怡, 杨颖, 陈华红, 等. 植物内生菌资源[J]. 微生物学通报, 2005, 32(6): 146-147.
[5]  Kobayashi D Y, Palumbo J D. Bacterial endophytes and their effects on plants and uses in agriculture[A]. In: Bacon C W, White J F. Microbial Endophytes[M]. New York: Jr. Marcel Dekker, Inc., 2000: 199-237
[6]  王刚, 李志强. 小麦内生细菌的分离及其对小麦纹枯病的拮抗作用[J]. 微生物通报, 2005, 32(2): 20-24.
[7]  罗明, 芦云, 张祥林. 棉花内生细菌的分离及生防益菌的筛选[J]. 新疆农业科学, 2004, 41(5): 277-282.
[8]  宋子红, 丁立孝, 马伯军, 等. 花生内生菌的种群及动态分析[J]. 植物保护学报, 1999, 26(4): 309-314.
[9]  周婧, 高增贵, 何秀玲, 等. 甜瓜枯萎病拮抗内生细菌筛选研究[J]. 北方园艺, 2008, (2): 225-227.
[10]  Fernando E V, Monica P R, Francisco P, et al. Endophytic bateria in Coffea arabica L.[J]. Journal of Basic Microbiology, 2005, 45(5): 371-380.
[11]  付业勤, 蔡吉苗, 刘先宝, 等. 香蕉内生细菌分离、活性评价及数量分布[J]. 热带作物学报, 2007, 28(4): 78-83.
[12]  Angela S, Birgit R, Gabriele B. Endophytic bacterial communities of field-grown potato plants and their plant-growth-promoting and antagonistic abilities[J]. Microbiology, 2004, 50: 239-249.
[13]  彭细桥, 周国生, 邓正平, 等. 烟草青枯病内生拮抗菌的筛选、鉴定及其防效测定[J]. 植物病理学报, 2007, 37(6): 670-674.
[14]  孔庆科, 丁爱云. 内生细菌作为生防因子的研究进展[J]. 山东农业大学学报(自然科学版), 2001, 32(2): 256-260.
[15]  李志真. 杨梅根瘤内生菌的生物学特性[J]. 林业科学, 2009, 45(1): 81-87.
[16]  史应武, 娄恺, 李春. 天山北坡甜菜内生菌分离鉴定及其动态变化[J]. 生态学报, 2009, 29(5): 2375-2382.
[17]  Berg G, Krechel A, Ditz M, et al. Endophytic and ectophytic potato-associated bacterial communities differ in structure and antagonistic function against plant pathogenic fungi[J]. FEMS Microbiology Ecology, 2005, 51(2): 215-229.
[18]  黄贵修, Yuka T, Segenet K. 臂形草内生菌特异DNA片段的克隆及其分子鉴定[J]. 热带作物学报, 2002, 23(4): 58-61.
[19]  Ahlholm J U, Helander M, Henriksson J, et al. Environmental conditions and host genotype direct genetic diversity of venturia ditricha, a fungal endophyte of birch trees[J]. Evolution, 2002, 56(8): 1566-1573.
[20]  谢永芳, 梁亦龙, 王会会, 等. 大蒜内生菌抑菌蛋白提取研究[J]. 江苏农业科学, 2009, (1): 122-123.
[21]  Sturz A V, Christie B R, Nowak J. Baterial endophytes: Potential role in developing sustainable systems of crop production[J]. Critical Reviews in Plant Sciences, 2000, 19(1): 1-30.
[22]  黄东益, 黄小龙, Segenet K. 旗草内生真菌与旗草抗病性研究[J]. 草业学报, 2009, 18(2): 39-45. 浏览
[23]  黄东益, 黄小龙. 禾本科牧草内生真菌的研究与应用[J]. 草业学报, 2008, 17(3): 128-136.
[24]  Siciliano S D, Germida J J. Taxonomic diversity of bacteria associated with the roots of field-grown transgenic Brassica napus cv. Quest, compared to the non-transgenic B. napus cv. Excel and B. rapus cv. Parkland[J]. FEMS Microbiology Ecology, 1999, 29(3): 263-272.
[25]  李倍金, 罗明, 周俊, 等. 几种禾草内生固氮菌的分离及固氮活性测定[J]. 草业学报, 2008, 17(5): 37-42. 浏览
[26]  Clay K, Holah J. Fungal endophyte symbiosis and plant diversity in successional fields[J]. Science, 1999, 285: 1742-1745.
[27]  Peters S, Draeger S, Aust H J, et al. Interactions in dual cultures of endophytic fungi with host and nonhost plant calli[J]. Mycologia, 1998, 90: 360-367.
[28]  刘志辉, 蔡杏姗, 竺澎波, 等. 应用气相色谱技术分析全细胞脂肪酸快速鉴定分枝杆菌[J]. 中华结核和呼吸杂志, 2005, 28(6): 403-406.
[29]  张国赏, 吴文娟, 潘仁瑞. 气相色谱-质谱法检测细胞脂肪酸及其在细菌鉴定上的应用[J]. 合肥联合大学学报, 2000, 10(4): 92-96.
[30]  Riley M S, Cooper V S, Lenski R E, et al. Rapid phenotypic change and diversification of a soil bacterium during 1000 generations of experimental evolution[J]. Microbiology, 2001, 147: 995-1006.
[31]  Ozbek A, Aktas O. Identification of three strains of Mycobacterium species isolated from clinical samples using fatty acid methyl ester profiling[J]. The Journal of International Medical Research, 2003, 31(2): 133-140.
[32]  Muller K, Schmid E N, Kroppenstedt R M. Improved identification of mycobacteria by using the microbial identification system in combination with additional trimethylsulfonium hydroxide pyrolysis[J]. Journal of Clinic Microbiology, 1998, 36(9): 2477-2480.
[33]  Basile F, Voorhces K J, Hadtleld T L. Microorganism gram-type differentiation based on pyrolysis mass spectrometry of bacterial fatty acid methyl ester extracts[J]. Applied and Environmental Microbiology, 1995, 61(4): 1534-1539.
[34]  王瑶瑶, 韩烈保, 曾会明. 禾本科植物内生菌研究进展[J]. 生物技术通报, 2008, (3): 33-38.
[35]  韦红群, 邓建珍, 曹建华, 等. 柱花草根系与根际微生物类群的研究[J]. 草业科学, 2009, 26(1): 69-73.
[36]  朱育菁, 陈璐, 蓝江林, 等. 茶叶内生菌的分离鉴定及其生防功能初探[J]. 福建农林大学学报(自然科学版), 2009, 38(2): 129-134.
[37]  朱育菁, 王秋红, 陈璐, 等. 龙眼内生菌的分离与脂肪酸鉴定[J]. 亚热带植物科学, 2008, 37(4): 22-25.
[38]  Cabral C M, Cherqui A, Pereira A, et al. Purification and characterization of two distinct metalloproteases secreted by the entomopathogenic bacterium photorhabdus sp. strain Az29[J]. Applied and Environmental Microbiology, 2004, 70: 3831-3838.
[39]  Mariёt J V D W, Henk J S, Jan A M B. Rhodococcus erythropolis DCL14 contains a novel degradation pathway for limonene[J]. Applied and Environmental Microbiology, 1999, 65(5): 2092-2102.
[40]  Bernardo V R, Jacquie M M, Robert A C, et al. Susceptibility of calves to challenge with Salmonella typhimurium 4/74 and derivatives harbouring mutations in htrA or purE[J]. Microbiology, 2000, 146(11): 2775-2783.
[41]  Wolf A, Fritze A, Hagemann M, et al. Stenotrophomonas rhizophila sp. nov., a novel plant-associated bacterium with antifungal properties[J]. International Journal of Systematic and Evolutionary Microbiology, 2002, 52: 1937-1944.
[42]  Fisher P J, Petrini O, Scott H M. The distribution of some fungal and bacterial endophytes in maize (Zea mays L.)[J]. New Phyto1ogist, 1992, 122: 299-305.
[43]  马同锁, 田苗珍, 袁红楼, 等. 不同生长环境对2种蔬菜内生菌分布的影响[J]. 安徽农业科学, 2009, 37(17): 7812-7813, 7815.

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