全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
草业学报  2010 

草地早熟禾新格莱德胚性愈伤组织原生质体培养及植株再生的研究

, PP. 55-60

Keywords: 草地早熟禾,新格莱德,原生质体培养,植株再生

Full-Text   Cite this paper   Add to My Lib

Abstract:

以草地早熟禾品种——新格莱德成熟种子为供试材料,在含3.0mg/L2,4-二氯苯氧乙酸(2,4-D)、0.5mg/L6-苄氨基嘌呤(6-BA)的MB5培养基中进行胚性愈伤组织诱导的培养。并从生长了7~9个月的胚性愈伤组织中分离出原生质体,将该原生质体置于KM8P培养基(含3.0mg/L2,4-D、0.5mg/L6-BA、100mg/L水解酪蛋白、100mg/L水解乳蛋白、1%蔗糖、0.4mol/L甘露醇)中进行了液体浅层培养。结果表明,新格莱德原生质体在上述KM8P培养基中培养3d后出现第1次细胞分裂,2~3周后形成小细胞团,此时添加低渗培养液2~3次,小细胞团持续分裂并形成愈伤组织。当愈伤组织块长至3~5mm时,转入固体培养基MS+3.0mg/L2,4-D+0.5mg/L6-BA和MS+0.5mg/L萘乙酸(NAA)+5.0mg/L6-BA上进行培养,使其细胞增殖和分化,且逐步形成完整的植株。

References

[1]  Kirsten A N, Else L, Elisabeth K. Regeneration of protoplast- derived green plants of Kentucky bluegrass (Poa protensis L.)[J]. Plant Cell Reports, 1993, 12: 537-540.
[2]  王喆之, 张苏锋, 胡正海. 陆地棉培养愈伤组织原生质体的制备、培养及再生植株[J]. 植物学报, 1998, 40(3): 234-240.
[3]  朱志清. 植物细胞工程[M]. 北京: 化学工业出版社, 2003: 153.
[4]  Sun C S, Prioli L M, Sondahl M R. Regeneration of haploid and dihaploid plants from protoplasts of supersweet (sh2sh2) corn[J]. Plant Cell Reports, 1989, 8: 313-316.
[5]  蔡起贵, 郭伯琛, 钱迎倩. 棉花原生质体的制备、培养及再生植株[J]. 植物学报, 1987, 29: 453-458.
[6]  Terakawa T, Sato T, Koike M. Plant regeneration from protoplasts isolated from embryogenic suspension cultures of creeping bentgrass (Agrostis palustris)[J]. Plant Cell Reports, 1992, 11(9): 457-461.
[7]  Dalton S J. Plant regeneration from cell suspension protoplasts of Festuca arundinacea and Lolium perenne[J]. Journal of Plant Physiology, 1988, 132: 170-175.
[8]  Wang Z Y, Valles M P, Montavon P. Fertile plant regeneration fromprotoplasts of meadow fescue (Festuca pratensis)[J]. Plant Cell Reports, 1993, 12(2): 95-100.
[9]  Inokuma C, Cho C, Okawara R, et al. Plant regeneration from protoplasts of Japanese lawngrass[J]. Plant Cell Reports, 1996, 15(12): 737-741.
[10]  姜淑慧, 管荣展, 董海滨, 等. 播娘蒿愈伤组织原生质体培养体系的建立[J]. 草业学报, 2006, 15(4): 94-98.
[11]  王瑛华, 陈刚, 贾敬芬, 等. 霸王的原生质体培养及植株再生研究[J]. 草业学报, 2009, 18(3): 110-116.
[12]  于晓玲, 李春强, 彭明. 植物原生质体技术及其应用[J]. 中国农学通报, 2009, 25(8): 22-26.
[13]  刘强, 张宗申, 丛丽娜, 等. 黄芪原生质体分离技术[J]. 广西植物, 2008, 28(3): 411-413.
[14]  肖尊安, 沈德绪, 林柏年. 中华猕猴桃原生质体再生植株[J]. 植物学报, 1992, 34: 736-742.
[15]  Oliveira M M, Pais M S S. Plant regeneration from protoplasts of long-term callus cultures of Actinidia deliciosa var d eliciosa cv. Hayward (kiw ifruit)[J]. Plant Cell Reports, 1991, 9: 643-646.
[16]  Binding H, Gorschen E, Jorgensen J. Protoplast culture in agarose media with particular emphasis to streaky lenses[J]. Botanica Acta, 1988, 101: 233-239.
[17]  何道一, 孙山. 苹果单倍体原生质体培养再生植株[J]. 核农学报, 2004, 18(6): 411-415.
[18]  Vander Valk P, Zeal M A C M, Creemers-Molenaar J. Regeneration of albino plantlets from suspension culture derived protoplasts of Kentucky bluegrass (Poa protensis L.)[J]. Euphytica, 1988, 5: 169-176.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133