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草业学报 2011

一株纤维素分解菌的鉴定及对两种草坪草凋落物分解活性的研究

, PP. 170-179

芦光新,陈秀蓉,杨成德,薛莉, 刘雯,卞静

Keywords: 高寒草地土壤真菌,草坪草凋落物,细胞壁物质,分解活性

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Abstract:

对1株分离筛选自东祁连山高寒草地土壤真菌菌株F1的分类学地位及对多年生黑麦草和白三叶凋落物的分解活性进行了研究。采用形态学和rDNA-ITS分子生物学相结合的方法,对菌株F1的分类学地位进行了确定,初步鉴定为Microdochiumbolleyi;应用摇瓶液体发酵法,通过接种和不接种处理,分别以2种草坪草凋落物为底物,于25℃,150r/min震荡培养42d,测定了2种草坪草凋落物诱导真菌分泌木质纤维素酶活力动态变化和草坪草凋落物细胞壁物质(CWM)的降解率。结果表明,1)接种处理后,2种草坪草细胞壁非水溶性物质(ICWM)的量显著减少(P<0.05);2)漆酶出现最早,其次是纤维素酶和木聚糖酶,邻苯二酚氧化酶和愈创木酚氧化酶出现最晚;3)与白三叶凋落物为底物的发酵体系相比,黑麦草凋落物ICWM和CWM降解率高,且差异显著(P<0.05);4)除漆酶之外,以黑麦草凋落物诱导菌株F1分泌的滤纸酶、羧甲基纤维素酶、木聚糖酶、邻苯二酚氧化酶和愈创木酚氧化酶活力比白三叶诱导相应的酶活力高(P<0.05)。

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