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草业学报  2011 

羊草乙醛脱氢酶基因LcALDH的克隆与表达分析

, PP. 187-193

Keywords: 羊草,乙醛脱氢酶,抗逆性

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Abstract:

采用RACE技术从羊草中克隆了乙醛脱氢酶基因LcALDH的cDNA(GenBank登录号EF492045)。长为1712bp的LcALDHcDNA序列含有编码500个氨基酸的1503bp的开放读码框、66bp的5′非翻译区和144bp的3′非翻译区。氨基酸序列中含有醛脱氢酶家族绝对保守的谷氨酸活性位点和半胱氨酸残基活性位点。分析LcALDH基因在不同胁迫条件下的表达情况,结果表明,LcALDH的表达受到低温、干旱、高盐和ABA的正调控作用。研究结果为进一步从分子水平探明羊草的抗逆机制,挖掘并利用植物抗逆基因奠定基础。

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