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草业学报  2011 

不同防褐化措施对苏丹草愈伤诱导以及抗褐化的效果研究

, PP. 174-181

Keywords: 苏丹草,愈伤诱导,防褐化措施

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Abstract:

苏丹草组织材料在组织培养过程中会产生酚类物质,影响愈伤组织的生长,甚至导致愈伤组织死亡。本试验以苏丹草幼穗为外植体,探讨了不同防褐化措施对苏丹草愈伤诱导的影响及抗褐化的效果防止。结果表明,活性炭、VC、PVP和AgNO3均能不同程度地提高愈伤诱导率和降低褐化率。其中,培养基中低浓度的蔗糖(10~20g/L)能降低褐化率,但同时也降低了愈伤诱导率,而高浓度的蔗糖(40~50g/L)能提高愈伤诱导率但同时也增加了褐化率。柠檬酸能提高愈伤诱导率但不能防止褐化。当活性炭的添加量为0.15g/L时,愈伤的诱导及褐化防止效果最好。

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