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草业学报  2011 

采用流式细胞仪鉴定紫花苜蓿花药愈伤组织的变异

, PP. 156-161

Keywords: 苜蓿,花药愈伤组织,流式细胞仪,DNA含量变异,遗传变异

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Abstract:

本试验采用流式细胞仪,对3个基因型8个继代周期的苜蓿愈伤组织DNA含量进行测定。通过DPAC软件(datapoolapplicationofcytometre)分析出细胞DNA含量变异百分率和处于S期细胞的百分率,并采用SPSS10.0软件分析出愈伤组织继代时间与DNA含量变异率之间的关系。结果表明,本试验所采用的3个基因型8个继代周期24个紫花苜蓿愈伤组织样本DNA含量均发生了变化,且全都出现了DNA加倍的现象。在24个样本中,DNA含量变异率最大的是自选系大叶0510的愈伤组织继代15周时的样本,变异率达到了19.45%。通过SPSS10.0软件分析,表明紫花苜蓿愈伤组织DNA含量变异细胞百分率与继代时间存在相关性,随着继代时间的延长,染色体变异率有增加的趋势,但这种趋势不是无限增大的。继代0~12周是愈伤组织DNA含量变异的集中发生期,以后随着继代次数的增加,DNA含量变异率趋向于稳定。

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