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草业学报  2011 

多浆旱生植物霸王液泡膜H+-PPase基因片段的克隆及序列分析

, PP. 119-124

Keywords: 霸王,H+-PPase基因,克隆,序列分析

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Abstract:

根据其他植物H+-PPase基因的保守序列设计一对简并性引物,以霸王叶片总RNA为模板,采用RT-PCR的方法扩增出H+-PPase基因片段并克隆到PUCm-T载体。阳性克隆经PCR鉴定后进行测序,序列分析结果表明,克隆到3个同源的序列片段(898bp),编码299个氨基酸;所得序列与GenBank中注册的高等植物H+-PPase基因核苷酸序列的同源性均在69%以上、氨基酸序列的同源性达78%以上。

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