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草业学报  2011 

腐殖质在ComamonaskoreensisCY01介导的2,4-二氯苯氧乙酸还原脱氯过程中的作用

, PP. 248-252

Keywords: 2,4-二氯苯氧乙酸,Comamonaskoreensis,还原脱氯

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Abstract:

从广东四会原始森林土壤中分离到一株能高效还原Fe(III)和腐殖质(HS)的兼性厌氧菌,编号为CY01,该菌株被鉴定为Comamonaskoreensis(韩国丛毛单胞菌)。以ComamonaskoreensisCY01为研究对象,研究了厌氧条件下HS(腐殖质)的模式物AQDS对微生物介导的2,4-D(2,4-二氯苯氧乙酸)还原脱氯过程的影响。研究结果显示,1)CY01具有使2,4-D进行脱氯降解的能力,CY01引起的2,4-D的脱氯降解过程是伴随着电子供体葡萄糖的氧化同时进行的;2)反应体系中加入AQDS,会促进2,4-D的降解过程,AQDS在Fe(III)/HS还原菌和2,4-D之间充当电子穿梭体。综合以上实验结果表明,CY01菌株引起的2,4-D的还原脱氯过程的完成是随着电子供体的氧化产生电子,然后AQDS将产生的电子加速传递至2,4-D,使2,4-D发生还原脱氯降解。

References

[1]  Coates J D. Humic substances as a mediator for microbially catalyzed metal reduction. Acta Hydrochimicaet Hydrobiologica, 1998, 26: 152-157. 3.0.CO;2-D target="_blank">
[2]  Fultz M L, Durst R A. Mediator compounds for the electrochemical study of biological redox systems. Analytica Chimica Acta, 1982, 14: 1-18.
[3]  Lovley D R. Bioremediation of organic and metal contaminants with dissimilatory metal reduction. Journal of Industrial MicrobioIogy &Biotechnology, 1995, 14: 85-93.
[4]  Lovley D R. Microbial reduction of iron manganese and other metals. Advances in Agronomy, 1995, 54: 175-231.
[5]  Lovley D R, Coates I D, Saffarini D A, et al. Dissimilatory iron reduction. In: Winkelman, Carrano C. Iron and Related Transition Metals in Microbial Metabolism. Switzerland: Harwood Academic Publishers, 1997: 187-215.
[6]  Busa J S, Hammond L E. Regulatory progress, toxicology, and public concerns with 2, 4-D: Where do we stand after two decades. Crop Protection, 2007, 26: 266-269.
[7]  李小坤, 鲁剑巍, 陈防. 牧草施肥研究进展. 草业学报, 2008, 17(2): 136-142.
[8]  Neilson J W, Josephson K L, Pillai S D, et al. Polymerase chain reaction and gene probe detection of the 2, 4-dichlorophenoxyacetic acid degradation plasmid, pJP4. Applied & Environmental Microbiology, 1992, 58: 1271-1275.
[9]  周萍, 刘国彬, 薛萐. 草地生态系统土壤呼吸及其影响因素研究进展. 草业学报, 2009, 18(2): 184-193. 浏览
[10]  Curtis G P, Reinhard M. Reductive dehalogenation of hexachloroethane, carbon tetrachloride, and bromoform by anthrahydroquinone disulfate and humic acid. Environmental Science & Technology, 1994, 28: 2393-2401.
[11]  Lovley D R, Coates J D, Blunt-Harris E L, et al. Humic substances as electron acceptors for microbial respiration. Nature, 1996, 382: 445-448.
[12]  Cervantes F J, Lettinga L, Vu-Thi-Thu G, et al. Quinone-respiratio improves dechlorination of carbon tetrachloride by anaerobic sludge. Applied Microbiology Biotechnology, 2004, 64: 702-711.
[13]  Hong Y G, Guo J, Xu Z C, et al. Humic substances act as electron acceptor znd redox mediator for microbial dissimilatory azoreduction by Shewanella decolorationis S12. Journal of Microbiology and Biotechnology, 2007, 17: 428-437.
[14]  Straub K L, Schink B. Evaluation of electron-shuttling compounds in microbial ferric iron reduction. FEMS Microbiology Letters, 2003, 220: 229-233.
[15]  Apper L, Mcknight D, Robinfulton J, et al. Fulvic acid oxidation state detectionusing fluorescence spectroscopy. Environmental Science & Technology, 2002, 36: 3170-3175.
[16]  Miller G L. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 1959, 31: 426-428.
[17]  王弋博, 武春媛, 周顺桂. 韩国丛毛单胞菌CY01的铁还原和腐殖质还原. 兰州大学学报(自然科学版), 2010, 46(2): 35-39.
[18]  Leahy J G, Colwell R R. Microbial degradation of hydrocarbons in the environment. Microbiology Molecular Biology Reviews, 1990, 54: 305-315.
[19]  Coates J D, Ellis D J, Blunt-Harris E L, et al. Recovery of humic-reducing bacteria from a diversity of environments. Applied & Environmental Microbiology, 1998, 64: 1504-1509.
[20]  Kristina L S, Andreas K, Bernhard S. Enrichment and isolation of ferric-iron- and humic-acid-reducing bacteria. Methods in Enzymology, 2005, 397: 58-70.
[21]  Lovley D R, Frage J L, Blunt-Harris E L, et al. Humic substances as a mediator for microbially catalyzed metal reduction. Acta Hydrochimicaet Hydrobiologica, 1998, 26: 152-157. 3.0.CO;2-D target="_blank">
[22]  Loffler F E. Enrichment, cultivation, and detection of reductively dechlorinating bacteria. Methods in Enzymology, 2005, 397: 77-111.

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