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草业学报  2012 

苜蓿乙烯应答因子基因的表达特性和生物信息学分析

, PP. 166-174

Keywords: 紫花苜蓿,乙烯应答因子,生物信息学分析,半定量RT-PCR,表达分析

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Abstract:

乙烯应答因子(ethyleneresponsefactor,ERF)是植物特有的一类转录因子,参与植物生长发育和胁迫应答等多个生理生化过程。本研究以拟南芥AP2结构域为探针搜索美国国立生物技术信息中心(NCBI)的苜蓿属蛋白质数据库,运用生物信息学软件分析苜蓿乙烯应答因子的系统进化、疏水轮廓、二级结构、信号肽以及亚细胞定位情况。运用半定量RT-PCR技术研究5个紫花苜蓿乙烯应答因子基因(MsERF1,MsERF2,MsERF6,MsERF7,MsERF9)表达的组织差异性以及多种处理条件下的表达特性。结果表明,5个乙烯应答因子基因在叶中的表达量都明显高于其他组织,不同基因有不同的组织表达特性;NaCl和PEG6000对MsERF2的表达没有影响,但Al2(SO4)3能诱导其表达,其余基因的表达都受这3种处理的诱导;脱落酸对MsERF2的表达没有影响,能够诱导其他基因的表达;生长素只能诱导MsERF6的表达,对其他基因的表达没有影响;赤霉素、茉莉酸甲酯和乙烯利对5个基因的表达都起促进作用。本研究为进一步研究苜蓿乙烯应答因子的结构和功能奠定了基础,同时为研究不同信号传导途径之间的相互作用提供了依据。

References

[1]  Liu Q, Zhang G Y, Chen S Y. Structure and regulatory function of plant transcription factors. Chinese Science Bulletin, 2001, 46(4): 271-278.
[2]  Jack K, Okamur O, Caster B, et al. The AP2 domain of APETALA2 defines a large new family of DNA binding protein in Arabidopsis. Proceedings of the National Academy of Sciences, USA, 1997, 94(6): 7076-7081.
[3]  Nakano T, Suzuki K, Fujimura T, et al. Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiology, 2006, 140(2): 411-432.
[4]  Yamaguchi-Shinozaki K, Shinozaki K. A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. The Plant Cell, 1994, 6(2): 251-264.
[5]  王舟, 刘建秀. DREB/CBF类转录因子研究进展及其在草坪草和牧草抗逆基因工程中的应用.草业学报, 2011, 20(1): 222-236. 浏览
[6]  刘晓静, 郝凤, 张德罡, 等. 抗冻基因CBF2表达载体构建及转化紫花苜蓿的研究.草业学报, 2011, 20(2): 193-200.
[7]  Hao D Y, Ohme-Takagi M, Sarai A. Unique mode of GCC box recognition by the DNA-binding domain of ethylene-responsive element-binding factor (ERF domain) in plant. The Journal of Biological Chemistry, 1998, 273(41): 26857-26861.
[8]  杨红善, 常根柱, 周学辉, 等. 美国引进苜蓿品种半湿润区栽培试验. 草业学报, 2010, 19(1): 121-127. 浏览
[9]  张改娜, 贾敬芬. 豌豆清蛋白1(PAl)基因的克隆及对苜蓿的转化. 草业学报, 2009, 18(3): 117-125. 浏览
[10]  Bleecker A B, Kende H. ETHYLENE: A gaseous signal molecule in plants. Annual Review of Cell and Developmental Biology, 2000, 16: 1-18.
[11]  Chang C. The ethylene signal transduction pathway in Arabidopsis: an emerging paradigm. Trends in Biochemistry Science, 1996, 21(4): 129-133.
[12]  Ecker J. The ethylene signal transduction pathway in plants. Science, 1995, 268: 667-675.
[13]  江腾, 林勇祥, 刘雪, 等. 苜蓿全基因组WRKY转录因子基因的分析. 草业学报, 2011, 20(3): 211-218.
[14]  Menke F L H, Champion A, Kijne J W, et al. A novel jasmonate- and elicitor-responsive element in the periwinkle secondary metabolite biosynthetic gene Str interacts with a jasmonate- and elicitor-inducible AP2-domain transcription factor, ORCA2. The EMBO Journal, 1999, 18: 4455-4463.
[15]  Van der Fits L, Memelink J. The jasmonate-inducible AP2/ERF-domain transcription factor ORCA3 activates gene expression via interaction with a jasmonate-responsive promoter element. The Plant Journal, 2001, 25(1): 43-53.
[16]  Zhang G Y, Chen M, Li L C, et al. Overexpression of the soybean GmERF3 gene, an AP2/ERF type transcription factor for increased tolerances to salt, drought, and diseases in transgenic tobacco. Journal of Experimental Botany, 2009, 60(13): 3781-3796.
[17]  Mauch-Mani B, Mauch F. The role of abscisic acid in plant-pathogen interactions. Current Opinion in Plant Biology, 2005, 8(4): 409-414.
[18]  Thara V K, Tang X Y, Gu Y Q, et al. Pseudomonas syringae pv tomato induces the expression of tomato EREBP-like genes Pti4 and Pti5 independent of ethylene, salicylate and jasmonate. The Plant Journal, 1999, 20(4): 475-483.
[19]  Liu Q, Kasuga M, Sakuma Y, et al. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought-and low-temperature-responsive gene expression, respectively, in Arabidopsis. The Plant cell, 1998, 10: 1391-1406.
[20]  Seo Y J, Park J B, Cho Y J, et al. Overexpression of the ethylene-responsive factor gene BrERF4 from brassica rapa increases tolerance to salt and drought in Arabidopsis Plants. Molecules and Cells, 2010, 30: 271-277.
[21]  Zhang H W, Liu W, Wan L Y, et al. Functional analyses of ethylene response factor JERF3 with the aim of improving tolerance to drought and osmotic stress in transgenic rice. Transgenic Research, 2010, 19: 809-818.
[22]  Zhang Z J, Li F, Li D J, et al. Expression of ethylene response factor JERF1 in rice improves tolerance to drought. Planta, 2010, 232: 765-774.

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