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草业学报  2012 

玉米持绿相关QTL整合图谱构建及一致性QTL区域内候选基因发掘

, PP. 175-185

Keywords: 玉米,持绿性,数量性状位点(QTL),整合图谱,元分析

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Abstract:

以玉米高密度遗传连锁图谱IBM22008Neighbors为参考图谱,收集来自不同实验中的173个玉米持绿相关数量性状位点(quantitativetraitlocus,QTL)信息,利用BioMercator2.1软件,构建出玉米持绿相关QTL整合图谱;采用元分析技术,在1,4,5,9号染色体上发掘出5个持绿“一致性”QTL区间。根据“一致性”QTL区间两端标记在玉米物理图谱B73RefGen_v2上的位置,将“一致性”QTL区间进行物理图谱定位,利用PlantGDB(http://www.plantgdb.org/)在线区段批量下载工具(downloadregiondata)下载“一致性”区间的1445个预测基因序列并进行生物信息学分析,发现预测基因主要参与具体的细胞过程,执行结合功能,催化、转移酶活性和氧化还原酶活性等分子功能。根据“一致性”QTL区间的基因位点名称,在NCBI中下载相关基因序列,与所在“一致性”QTL区间所有预测基因保守结构域进行比对,在5个“一致性”持绿QTL区间内初步确定8个持绿相关候选基因。利用GRAMENE网站(http://www.gramene.org/)的Cmap功能,将水稻持绿基因sgr(staygreen)转定位于玉米物理图谱B73RefGen_v2上,找到与其同源的玉米候选基因GRMZM2G091837_T01,其序列与已发表的玉米衰老诱导叶绿体持绿蛋白基因sgr1序列一致。

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