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草业学报  2012 

多花黑麦草品种间杂交F2代分子标记遗传分析

, PP. 125-133

Keywords: 多花黑麦草,杂交F2代,遗传关系,SRAP,SSR

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Abstract:

运用序列相关扩增多态性(SRAP)和微卫星标记(SSR)技术对6个多花黑麦草亲本品种及其9个杂交F2代组合80份材料进行遗传分析。筛选出具有多态性的15对SRAP引物、16对SSR引物,SRAP标记平均每对引物扩增出16.7条带,多态性位点百分率为68.88%。SSR标记平均每对引物扩增出14.75条带,多态性位点百分率为58.05%。通过聚类树状图分析得出结论,杂交F2代内同一组合基本聚为一类,长江2号×剑宝和长江2号×阿伯德组合除外;父本和母本与其杂交F2代遗传距离与聚类结果基本一致。同时还发现SRAP标记对杂交F2代及亲代聚类分析可靠性高于SSR标记。

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