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草业学报  2012 

披碱草内生真菌共生体中麦角生物碱的组织分布与季节动态

, PP. 84-92

Keywords: 披碱草,内生真菌,麦角生物碱,分布,季节动态

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Abstract:

采用高效液相色谱(HPLC)检测了2009年4,6,8和10月采集的雾灵山、沁源、五台山和百望山等4个侵染了Neotyphodium属内生真菌的披碱草种群的叶片、茎、叶鞘和根以及10月采集的种子中2种麦角生物碱(麦角酰胺和麦角新碱)的含量。结果表明,在4,6和8月,4个披碱草种群的麦角酰胺主要分布在叶片(5.037~30.037mg/kg)和叶鞘(6.259~22.255mg/kg),10月,麦角酰胺主要集中在种子(5.045~8.971mg/kg)和叶片(3.119~6.658mg/kg);各种群的叶片、茎秆和叶鞘中麦角酰胺在4或6月含量(13.831~30.037mg/kg)最高,8或10月含量(3.119~6.658mg/kg)显著减小(P<0.05),根中8月麦角酰胺(0.552~0.576mg/kg)显著低于其他月份(P<0.05)。麦角新碱含量的分布与麦角酰胺相似,4,6和8月主要分布于叶片(2.206~8.226mg/kg)和叶鞘(0.268~5.490mg/kg)中,10月主要集中于种子(1.647~5.664mg/kg)和叶片(1.353~2.251mg/kg)中;各种群的叶片、茎秆和叶鞘中麦角新碱在4或6月(5.102~8.226mg/kg)最高,8或10月含量(1.353~2.251mg/kg)显著减小(P<0.05),根中麦角新碱在8月含量(0.128~0.283mg/kg)低于其他月份。不同生长时期,4个披碱草种群整株中麦角酰胺和麦角新碱的含量均不同。麦角生物碱的总含量中麦角酰胺显著高于麦角新碱(P<0.05),麦角酰胺和麦角新碱呈极显著线性正相关(P<0.01)关系,相关系数为r=0.827。

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