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草业学报  2012 

中国新疆紫花苜蓿复合体3个种的遗传多样性及亲缘关系研究

, PP. 190-198

Keywords: 紫花苜蓿复合体,遗传多样性,亲缘关系

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Abstract:

利用15对SSR引物和10个ISSR引物对紫花苜蓿复合体3个种黄花苜蓿、多变苜蓿及紫花苜蓿共10个居群的遗传多样性及亲缘关系进行研究。结果表明,SSR标记下,10个居群中多变苜蓿VG居群多态位点百分率、Shannon信息指数及Nei’s基因多样性指数均最高;ISSR标记下,黄花苜蓿FH居群以上3个指数均最高;黄花苜蓿居群FH在2种分子标记下都存在特有位点。说明在10个居群中多变苜蓿居群VG与黄花苜蓿居群FH表现出较丰富的遗传多样性,值得进一步保护。UPGMA聚类分析结果表明,在2种分子标记下3个种可以被有效区分,结合它们在新疆境内的分布,本研究支持仍将紫花苜蓿、黄花苜蓿和多变苜蓿划分为3个种。

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