全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
草业学报  2012 

牦牛尿嘌呤衍生物排出量对皱胃注射酵母RNA水平的响应

, PP. 248-253

Keywords: 牦牛,嘌呤衍生物,皱胃,微生物氮

Full-Text   Cite this paper   Add to My Lib

Abstract:

本研究对3头牦牛施以皱胃瘘管手术,以酵母RNA为嘌呤碱基供体,连续注射4期,以期测定牦牛吸收嘌呤的回收率,为尿嘌呤衍生物估测牦牛瘤胃微生物氮产量的模型积累数据。结果表明,牦牛皱胃连续注射酵母RNA可使尿囊素(564~1426μmol/kgBW0.75)、总嘌呤衍生物(purinederivative,PD)排出量(629~1507μmol/kgBW0.75)及尿囊素占总PD比例线性提高(0.90~0.95)(P<0.01);嘌呤碱基注射水平对尿酸、肌酐及尿氮排出量影响不显著(P>0.05)。回归分析发现,牦牛皱胃嘌呤注射量(X,mmol/d)与尿嘌呤衍生物排出量(Y,mmol/d)间存在线性关系:Y=0.85X+33.02(R2=0.96,P<0.001),牦牛吸收嘌呤在尿中的回收率为85%。

References

[1]  Orellana B P, Balcells J, Martn-Orúe S M, et al. Excretion of purine derivatives in cows: Endogenous contribution and recovery of exogenous purine bases. Livestock Production Science, 2001, 68(3): 243-250.
[2]  Smith R H, McAllan A B. Nucleic acid metabolism in the ruminant: Amounts of nucleic acids and total and ammonia nitrogen in digesta from the rumen, duodenum and ileum of calves. British Journal of Nutrition, 1971, 25(1): 181-190.
[3]  McAllan A B. The fate of nucleic acids in ruminants. Proceedings of the Nutrition Society, 1982, 41(3): 309-316.
[4]  Chen X B, Gomes M J. Estimation of Microbial Protein Supply to Sheep and Cattle Based on Urinary Excretion of Purine Derivatives-an Overview of the Technical Details. Bucksburn Aberdeen: International Feed Resources Unit, 1992.
[5]  Ellis W C, Pfander W H. Rumen microbial polynucleotide synthesis and its possible role in ruminant nitrogen utilization. Nature, 1965, 205(4975): 974-975.
[6]  Leng R A, Nolan J V. Nitrogen metabolism in the rumen. Journal of Dairy Science, 1984, 67(5): 1072.
[7]  常祺, 刘满香. 青藏高原牦牛竞争力研究报告. 青海畜牧兽医杂志, 2002, 32(6): 22-25.
[8]  柴永清, 曹致中. 草地农业生态系统4个生产层理论对肃北县畜牧业可持续发展的指导. 草业科学, 2010, 27(4):160-164.
[9]  周刊社, 杜军, 袁雷, 等. 西藏怒江流域高寒草甸气候生产潜力对气候变化的响应. 草业学报, 2010, 19(5): 17-24. 浏览
[10]  李东, 黄耀, 吴琴, 等. 青藏高原高寒草甸生态系统土壤有机碳动态模拟研究. 草业学报, 2010, 19(2): 160-168. 浏览
[11]  淡瑞芳, 张海涛, 龙瑞军, 等. 藏系绵羊瘤胃细菌数量及其放牧地牧草养分随季节变化的研究. 草业学报, 2009, 18(1): 100-104. 浏览
[12]  韩兴泰, 胡令浩. 生长牦牛能量代谢的研究. 青海畜牧业, 1993, (1): 13-16.
[13]  胡令浩, 谢敖云. 不同海拔高度下生长牦牛绝食代谢的研究. 青海畜牧兽医杂志, 1992, 22(2): 1-5.
[14]  董世魁, 龙瑞军. 不同采食水平下舍饲干奶牦牛能量转化, 氮、钙和磷代谢的研究. 草业学报, 2000, 9(2): 32-37.
[15]  韩兴泰, 胡令浩. 粗饲条件下生长牦牛能量代谢的估测. 青海畜牧兽医杂志, 1992, 22(2): 21-22.
[16]  韩兴泰, 胡令浩, 谢敖云, 等. 牦牛瘤胃细菌中核糖核酸含量及其与细菌总氮比值的研究. 动物营养学报, 1998, 10(2): 35-39.
[17]  李林, 薛白. 控释尿素对牦牛瘤胃氨氮和微生物蛋白质的影响. 中国草食动物, 2007, 27(3): 10-11.
[18]  胡令浩, 谢敖云. 生长期牦牛瘤胃消化代谢的研究: II. 饲喂氨化麦秸对牦牛瘤胃代谢的影响. 青海畜牧兽医杂志, 1989, (3): 11-13.
[19]  薛白, 柴沙驼. 生长期牦牛蛋白质需要量的研究. 青海畜牧兽医杂志, 1994, 24(4): 1-4.
[20]  Ding L M, Long R J, Shang Z H, et al. Feeding behaviour of yaks on spring, transitional, summer and winter pasture in the alpine regionof the Qinghai-Tibetan plateau. Applied Animal Behaviour Science, 2008, 111: 373-390.
[21]  Wang H C, Long R J, Zhou W, et al. A comparative study on urinary purine derivative excretion for yak (Bos grunniens), cattle (Bos taurus) and crossbred (Bos grunniens×Bos taurus) in Qinghai-Tibetan plateau. Journal of Animal Science, 2009, 87:2355-2362.
[22]  Shao B P, Long R J, Ding Y P, et al. Morphological adaptations of yak (Bos grunniens) tongue to the foraging environment of the Qinghai-Tibetan Plateau. Journal of Animal Science, 2010, 88: 2594-2603.
[23]  Topps J H, Elliot R C. Relationship between concentrations of ruminal nucleic acids and excretion of purine derivatives by sheep. Nature (London), 1965, 2005: 498-499.
[24]  Verbic J, Chen X B, MacLeod N A, et al. Effect of microbial nucleic acid infusion on purine derivative excretion by steers. Journal of Agricultural Science, 1990, 114(3): 243-248.
[25]  Chen X B, Hovell F D, rskov E R, et al. Excretion of purine derivatives by ruminants: effect of exogenous nucleic acid supply on purine derivative excretion by sheep. British Journal of Nutrition, 1990, 63(1): 131-142.
[26]  Balcells J, Guada J A, Castrillo C, et al. Urinary excretion of allantoin and allantoin precursors by sheep after different rates of purine infusion into the duodenum. Journal of Agriculture Science, 1991, 116(2): 309-317.
[27]  Fujihara T, rskov E R, Reeds P J, et al. The effect of protein infusion on urinary excretion of purine derivatives in ruminants nourished by intragastric nutrition. Journal of Agricultural Science, 1987, 109(1): 7-12.
[28]  Antoniewicz A M, Pisulewski P M. Measurements of endogenous allantoin excretion in sheep urine. Journal of Agricultural Science, 1982, 98: 221-223.
[29]  Perez J F, Rodriguez C A, Gonzalez J, et al. Contribution of dietary purine bases to duodenal digesta in sheep. Animal Feed Science Technology, 1996, 62(4): 251-262.
[30]  Belenguer A, Yanez D, Balcells J, et al. Urinary excretion of purine derivatives and prediction of rumen microbial outflow in goats. Livestock Production Science, 2002, 77(2): 127-135.
[31]  Liang J B, Matsumoto M, Young B A. Purine derivative excretion and ruminal microbial yield in Malaysian cattle and swamp buffalo. Animal Feed Science and Technology, 1994, 47(4): 189-199.
[32]  Pimpa O, Liang J B, Jelan Z A, et al. Urinary excretion of duodenal purine derivatives in Kedah-Kelantan cattle. Animal Feed Science and Technology, 2001, 92(3): 203-214.
[33]  Vercoe J E. Urinary allantoin excretion and digestible dry-matter intake in cattle and buffalo. Journal of Agricultural Science, 1976, 86(3): 613-615.
[34]  Liang J B, Young B A. Comparative energetic efficiencies of male Malaysian cattle and buffalo. Livestock Production Science, 1995, 41(1): 19-27.
[35]  Pimpa O, Liang J B, Balcells J Q. Absorption of nucleic acid from the small intestines of swamp buffaloes and zebu cattle. Silpakorn Uuniversity Science and Technology Journal, 2007, 1(1): 26-35.
[36]  龙瑞军. 青藏高原草地生态系统之服务功能. 科技导报, 2007, 25(9): 26-28.
[37]  Guerouali A, Gass Y E, Balcells J, et al. Urinary excretion of purine derivatives as an index of microbial protein synthesis in the camel (Camelus dromedarius). British Journal of Nutrition, 2004, 92(2): 225-232.
[38]  IAEA. Estimation of Basal Purine Excretion, in Estimation of Rumen Microbial Protein Production from Purine Derivatives in Urine. Vienna: International Atomic Energy Agency, 1997: 21-24.
[39]  李晓鹏, 周围, 王虎成, 等. 高效液相色谱法对牦牛血浆与尿中嘌呤衍生物及肌酐含量的测定. 分析测试学报, 2009, 28(7): 867-861.
[40]  杨胜. 饲料分析及饲料质量检测技术. 北京:农业大学出版社, 1993.
[41]  Belenguer A, Yanez D, Balcells J, et al. Urinary excretion of purine derivatives and prediction of rumen microbial outflow in goats. Livestock Production Science, 2002, 77(3): 127-135.
[42]  Vagnoni D B, Broderick G A, Clayton M K, et al. Excretion of purine derivatives by Holstein cows abomasally infused with incremental amounts of purines. Journal of Dairy Science, 1997, 80(8): 1695-1702.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133