全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
草业学报  2012 

东方山羊豆脱水蛋白基因的克隆及初步分析

, PP. 176-183

Keywords: 东方山羊豆,脱水蛋白基因,基因克隆,实时荧光定量PCR,植物表达载体

Full-Text   Cite this paper   Add to My Lib

Abstract:

采用SMARTRACE方法,从东方山羊豆盐诱导抑制性差减杂交cDNA文库中分离到了一个脱水蛋白(GoDHN)基因。该基因cDNA全长1169bp,开放阅读框843bp,编码281个氨基酸,编码的蛋白质分子量为28.71kDa。经实时荧光定量PCR分析,GoDHN基因在东方山羊豆的茎和叶中表达量明显高于根中表达量,并且基因表达受ABA、NaCl和PEG的诱导,随着诱导时间的增加,表达量呈持续增长趋势。这些结果表明,DHN基因在东方山羊豆的抗逆性中可能起到重要的调控作用。本研究成功构建了pCAMBIA1302-DHN植物表达载体,为下一步转基因研究奠定了基础。

References

[1]  Allagulova C R, Gimalov F R, Shakirova F M, et al. The plant dehydrins: Structure and putative functions. Biochemistry (Moscow), 2003, 68(9): 945-951.
[2]  Dure L III, Crouch M, Harada J, et al. Common amino acid sequence domains among the LEA polypeptides of higher plants. Plant Molecular Biology, 1989, 12: 475-486.
[3]  Close T J. Dehydrins:emergence of a biochemical role of a family of plant dehydration proteins. Physiologia Plantarum, 1996, 97(4): 795-803.
[4]  Close T J. Dehydrins: A commonalty in the response of plants to dehydration and low temperature. Physiologia Plantarum, 1997, 100(2): 291-296.
[5]  Koag M, Fenton R D, Wilkens S, et al. The binding of maize DHN1 to lipid vesicles. Gain of structure and lipid specificity. Plant Physiology, 2003, 131: 309-316.
[6]  Kusano T, Aguan K, Abe M, et al. Nucleotide sequence of a rice rab16 homologue gene. Plant Molecular Biology, 1992, 18: 127-129.
[7]  Mundy J, Chua N H. Abscisic acid and water-stress induce the expression of a novel rice gene.Embo Journal, 1988, 7(8): 2279-2286.
[8]  Takahashi R, Joshee N, Kitagawa Y. Induction of chilling resistance by water stress, and cDNA sequence analysis and expression of water stress-regulated genes in rice. Plant Molecular Biology, 1994, 26(1): 339-352.
[9]  Yamaguchi-Shinozaki K, Mundy J, Chua N H. Four tightly linked rab genes are differentially expressed in rice. Plant Molecular Biology, 1990, 14(1): 29-39.
[10]  Duan X, Wang B, Hong B, et al. Expression of a late embryogenesis abundant protein gene, HVA1, from barely confers tolerance to water deficit and salt stress in transgenic rice. Plant Physiology, 1996, 110: 249-257.
[11]  Imai R, Chang L, Ohta A, et al. A LEA-class gene of tomato confers salt and freezing tolerance when expressed in Saccharomyces cerevisiae. Gene, 1996, 170(2): 243-248.
[12]  Zhang L, Ohta A, Takagi M, et al. Expression of plant group 2 and group 3 lea genes in Saccharomyces cerevisiae revealed functional divergence among LEA proteins. Journal of Biochemistry, 2000, 127(4): 611-616.
[13]  张清斌, 杨志忠, 贾纳提, 等. 东方山羊豆引种研究初报. 中国草地, 2001, 23(4): 17-20.
[14]  沈禹颖, 张自和, 谢田玲. 优良豆科牧草——东方山羊豆的研究与利用. 草业学报, 2003, 12(6): 105-109.
[15]  李鑫, 王赞, 王学敏, 等. 东方山羊豆液泡膜Na+/H+逆向转运蛋白基因的克隆与分析. 植物生理学通讯, 2009, 45(5): 444-448.
[16]  Chen X F, Wang Z, Wang X M, et al. Isolation and characterization of GoRAV, a novel gene encoding a RAV-type protein in Galegae orientalis. Genes & Genetic Systems, 2009, 84(2): 101-109.
[17]  梁哲, 姜三杰, 未丽, 等. 三叶草基因工程研究进展. 草业学报, 2009, 18(2): 205-211. 浏览
[18]  周玲玲, 缪建锟, 祝建波, 等. 大叶补血草Na+/H+逆向转运蛋白基因的克隆及序列分析. 草业学报, 2009, 18(5): 176-183. 浏览
[19]  Soulages J L, Kim K, Arrese E L. Conformation of a group 2 late embryogenesis abundant protein fromsoybean. Evidence of Poly (L-Proline)-type Ⅱ structure. Plant Physiology, 2003,131(3):963-975.
[20]  Kenneth J K, Thomas D S. Analysis of relative gene expression data using real-time quantitutive PCR and the 2-ΔΔCt method.Methods,2001,25:402-408.
[21]  Close T J, Kortt A A, Chandler P M. A cDNA-based comparison of dehydration-induced proteins (dehydrins) in barley and corn. Plant Molecular Biology, 1989, 13(1): 95-108.
[22]  Whitsitt M S, Collins R G, Mullet J E. Modulation of dehydration tolerance in soybean seedlings. Plant Physiology, 1997, 114(3): 917-925.
[23]  Kaye C, Neven L, Hofig A, et al. Characterization of a gene for spinach CAP160 and expression of two spinach cold-acclimation proteins in tobacco. Plant Physiology, 1998, 116(4): 1367-1377.
[24]  Artlip T S, Callahan A M, Bassett C L, et al. Seasonal expression of a dehydrin gene in sibling deciduous and evergreen genotypes of peach (Prunus persica Batsch). Plant Molecular Biology, 1997, 33(1): 61-70.
[25]  张玉秀, 王梓. 脱水蛋白在逆境下的分子作用机制研究进展. 自然科学进展, 2007, 17(1): 1-10.
[26]  Cellier F, Conejero G, Breitler J C, et al. Molecular and physiological responses to water deficit in drought tolerant and drought sensitive lines of sunflower. Plant Physiology, 1998, 116(1): 319-328.
[27]  Caruso A, Morabito D, Delmotte F, et al. Dehydrin induction during drought and osmotic stress in populus. Plant Physiology and Biochemistry, 2002, 40(12): 1033-1042.
[28]  张茹, 李金花, 柴兆祥, 等. Chi基因的克隆及转基因马铃薯植株的获得. 草业学报, 2009, 18(6): 51-58. 浏览

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133