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草业学报  2013 

苜蓿总黄酮对妊娠雌鼠繁殖性能及相关基因表达量的影响

DOI: 10.11686/cyxb20130631, PP. 249-256

Keywords: 苜蓿总黄酮,下丘脑-垂体-卵巢性腺轴,初生窝重,产仔数,大鼠,荧光定量

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Abstract:

为了研究苜蓿总黄酮对动物妊娠期间繁殖性能、繁殖器官、激素水平及下丘脑-垂体-卵巢性腺轴相关基因表达量的影响,本实验以雌性大鼠为模式动物,分为5个试验组:3个苜蓿总黄酮剂量组(120,400,1200mg/kg)和2个对照组(己烯雌酚0.5mg/kg、空白对照组),结果显示,苜蓿总黄酮中、高剂量组雌二醇(E2)显著升高(P<0.05),低剂量组E2极显著升高(P<0.01)。各试验组的血清中孕酮(P)含量不存在显著性差异(P>0.05)。苜蓿总黄酮3个剂量组的活胎数均显著提高(P<0.05),高剂量组的胎仔重极显著增重(P<0.01),而苜蓿总黄酮各剂量组的黄体数差异不显著,但存在随着剂量的增加而降低的趋势,己烯雌酚的黄体数与空白对照组相比显著减少(P<0.05)。苜蓿总黄酮3个剂量组的产仔数均显著高于空白对照组(P<0.05),而初生窝重则极显著高于对照组(P<0.01)。苜蓿总黄酮和己烯雌酚均显著降低下丘脑中GnRH的表达量(P<0.01),同时垂体的FSH表达量显著降低(P<0.01),卵巢中苜蓿总黄酮高剂量组的FSHR表达量显著降低(P<0.05),LHR表达量显著升高(P<0.05),其他基因表达量与空白对照相比差异不显著(P>0.05)。从以上可以看出,苜蓿总黄酮通过影响下丘脑-垂体-卵巢性腺轴,改善了妊娠期间雌鼠的繁殖性能,提高了产仔数和初生窝重。

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