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草业学报  2013 

花苜蓿内生放线菌多样性及群落结构

DOI: 10.11686/cyxb20130513, PP. 113-119

Keywords: 花苜蓿,放线菌,多样性,群落结构,DGGE

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Abstract:

为了了解药用植物花苜蓿内生放线菌的多样性和群落结构,将采自四川省甘孜地区的药用植物花苜蓿进行严格的表面消毒灭菌后,运用变性梯度凝胶电泳(denaturinggradientgelelectrophoresis,DGGE)技术对其根茎叶花等样品的内生放线菌进行分析。结果表明,花苜蓿不同组织中内生放线菌多样性和群落结构存在明显差异。其内生放线菌的多样性指数(H)和丰富度(S)均是花>根>茎>叶,而均匀度(EH)则是叶>根>茎>花。不同组织样品之间的放线菌群落结构相似性差异明显,其中茎与叶的相似性最高,根与叶之间相似性最低,其他组织之间相似性均很低。DGGE条带回收测序结果显示,大部分克隆条带属于链霉菌属(Streptomyces),但仍有超过43%的条带属于稀有放线菌,且主条带A23和共有条带A12属于稀有放线菌,说明部分稀有放线菌在花苜蓿样品内是优势放线菌。该研究表明了花苜蓿根茎叶花组织的内生放线菌存在丰富的多样性,且不同组织内生放线菌多样性存在明显差异,其中花组织多样性最丰富。说明甘孜地区的药用植物花苜蓿可作为一种较理想的分离内生放线菌的植物资源。

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