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草业学报  2013 

东祁连山线叶嵩草内生细菌X4的产吲哚乙酸、解磷、抗菌和耐盐特性研究及分子鉴定

DOI: 10.11686/cyxb20130417, PP. 137-146

Keywords: 内生细菌,普城沙雷氏菌,生物学,16SrDNA鉴定

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Abstract:

线叶嵩草内生细菌X4分离自东祁连山高寒草地,对其产吲哚乙酸、解磷、抗菌和耐盐特性及其分子生物学特性研究结果表明,其在含色氨酸的金氏培养基中分泌吲哚乙酸的量为50.74mg/L,溶磷圈直径/菌落直径(D/d)值分别为3.23和4.31,对辣椒立枯丝核病菌、黄瓜枯萎病菌、油菜菌核病菌及番茄灰霉病菌都有抑制作用,且致菌丝生长畸形,菌落生长的酸碱度范围为7~10,耐NaCl溶液范围为<7%,菌体短杆状,革兰氏阴性,无芽孢,16SrDNA序列与普城沙雷氏菌(Serratiaplymuthica)最为接近。该菌具有微生物杀菌剂、改良土壤等潜力。

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