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草业学报  2015 

4种暖季型草坪草币斑病病原菌鉴定及其生物学特性

DOI: 10.11686/cyxb20150116, PP. 124-131

Keywords: 暖季型草坪草,币斑病,Sclerotiniahomoeocarpa,生物学特性

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Abstract:

2011-2013年对海南省多个高尔夫球场及校园绿化草坪进行调查期间发现一种类似于草坪币斑病且可严重危害4种暖季型草坪草的草坪病害。根据科赫法则验证、形态学观察以及ITS序列分析的结果,确定引起海南省币斑病的病原菌为Sclerotiniahomoeocarpa,这是我国首次报道S.homoeocarpa危害杂交狗牙根和地毯草。生物学特性研究表明:S.homoeoparpa生长的适宜温度为25~30℃,最适温度为28℃;适宜pH值为4~6,最适pH值为5;菌丝的致死温度为55℃水浴处理10min;光照有利于菌丝生长。S.homoeoparpa能够利用多种碳氮源:碳源测试中,对可溶性淀粉利用率较高,对D-甘露醇和D-阿拉伯糖利用率较低;氮源测试中,病菌对硝酸铵、磷酸二氢铵的利用效果最佳,对脲利用效果最差。

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