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急性低氧大鼠脑31P核磁共振波谱研究

, PP. 105-112

Keywords: ,代谢,急性低氧,31P磁共振波谱

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Abstract:

31P磁共振波谱是目前唯一可以用作在体无损伤的检测细胞水平能量代谢变化的非侵入性技术,可测得脑内多种能量代谢产物.目的:急性低氧大鼠脑组织的31PMRS检测.方法:(1)20只成年SD大鼠分为4组:低氧0min(对照),5min,10min,15min后,迅速液氮冷冻;(2)将脑组织研碎后,加入高氯酸(PCA),冷冻干燥;(3)将提取物用0.5mLD\-2O溶解后进行MRS检测.结果:(1)急性低氧早期即引起31PMRS中PCr和ATP峰降低,ADP和Pi峰增高,PCr/Pi和ATP/Pi降低,而ADP/ATP增高.可交换磷池(EPP)中PCr的正常值为42.4%,低氧5min后降到28.9%,ATP从33.8%降到19.2%,Pi从17.7%升到42.0%.(2)急性低氧时31PMRS中脑内磷酯分解代谢产物GPC、GPE含量增加,说明低氧早期脑内即有膜磷酯的分解增加.结论:31P磁共振波谱可用于脑低氧性疾病的诊断,我们波谱中最敏感的指标是PCr/Pi和ATP/Pi,尤其早期降低更为显著.

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