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基于核磁共振的统计全相关谱在大鼠肾脏组织中的应用Hot!

, PP. 510-518

Keywords: NMR,统计全相关谱(STOCSY),高分辨魔角旋转,生物组织,肾脏

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Abstract:

生物组织是基于NMR的代谢组学研究的主要对象之一,广泛应用于分子病理学、毒理学、生物医学等众多领域.但是,为了保证测定的准确,组织的NMR实验往往需要在较低的温度下和较短时间内完成,以防止由于组织内酶的降解和扩散而导致的某些代谢物质的分析信息被破坏.统计全相关谱(StatisticalTotalCorrelationSpectroscopy,STOCSY)是依靠一维谱来实现二维谱的一些功能的方法,不需要额外的实验时间,已经被广泛应用于代谢组学研究中.本文采用STOCSY方法,通过对一系列1H高分辨魔角旋转谱的统计分析和计算,得到了肾脏组织的准二维相关谱,其中共振峰之间的相关较为准确的反应了物质之间的耦合信息,为物质的归属提供了帮助.

References

[1]  Tang H R, Wang Y L. Metabonomics: A revolution in progress[J]. Prog Biochem Biophys, 2006, 33(5): 401-417.
[2]  Lindon J C, Holmes E, Nicholson J K. Recent development and applications of NMR-based metabonomics[J]. Chinese J Magn Reson(波谱学杂志), 2006, 23(1): 101-127.
[3]  Nicholson J K, Lindon J C, Holmes E.′Metabonomics′: understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data[J]. Xenobiotica, 1999, 29(11): 1 181-1 189.
[4]  Constantinou M, Papakonstantinou E, Spraul M, et al. 1H MNR-based metabonomics for the diagnosis of inborn errors of metabolism in urine[J]. Anal Chim Acta, 2005, 542: 169-177.
[5]  Constantinou M, Papakonstantinou E, Benaki D, et al. Application of nuclear magnetic resonance spectroscopy combined with principal component analysis in detecting inborn errors of metabolism using blood Spots. A metabonomic approach[J]. Anal Chim Acta, 2004, 511: 303-312.
[6]  Moolenaar S H, Engelke U F, Wevers R A. Proton nuclear magnetic resonance spectroscopy of body fluids in the field of inborn errors of metabolism[J]. Ann Clin Biochem, 2003, 40(Pt 1): 16-24.
[7]  Lindon J C, Keun H C, Ebbels T M, et al. The consortium for metabonomic toxicology (COMET): aims, activities and achievements[J]. Pharmacogenomics, 2005, 6(7): 691-699.
[8]  Lindon J C, Nicholson J K, Holmes E, et al. Contemporary issues in toxicology the role of metabonomics in toxicology and its evaluation by the COMET project[J]. Toxicol Appl Pharm, 2003, 187(3): 137-146.
[9]  Bundy J G, Spurgeon D J, Svendsen C, et al. Earthworm species of the genus Eisenia can be phenotypically differentiated by metabolic profiling[J]. FEBS Lett, 2002, 521(1-3): 115-120.
[10]  Bundy J G, Lenz E M, Bailey N J, et al. Metabonomic assessment of toxicity of 4-fluoroaniline, 3,5-difluoroaniline and 2-fluoro-4-methylaniline to the earthworm Eisenia veneta (Rosa): identification of new endogenous biomarkers[J]. Environ Toxicol Chem, 2002, 21(9): 1 966-1 972.
[11]  Zhu Hang(朱航), Tang Hui-ru(唐惠儒), Zhang Xu(张许), et al. NMR-based metabonomics(基于NMR的代谢组学研究)[J]. Hua Xue Tong Bao(化学通报), 2006, 69: 1-9.
[12]  Yan Xian-zhong(颜贤忠), Zhao Jian-yu(赵剑宇), Peng Shuang-qing(彭双清), et al. Metabonomics in post-genomic era(代谢组学在后基因组时代的作用)[J]. Chinese J Magn Reson(波谱学杂志), 2004, 21(2): 263-271.
[13]  Vejchapipat P, Williams S R, Spitz L, et al. Intestinal metabolism after ischemia-reperfusion[J]. J Pediatr Surg, 2000, 35(5): 759-764.
[14]  Cheng L L, Chang I W, Smith B L, et al. Evaluating human breast ductal carcinomas with high-resolution magic-angle spinning proton magnetic resonance spectroscopy[J]. J Magn Reson, 1998, 135(1): 194-202.
[15]  Duarte I F, Stanley E G, Holmes E, et al. Metabolic assessment of human liver transplants from biopsy samples at the donor and recipient stages using high-resolution magic angle spinning 1H NMR spectroscopy[J]. Anal Chem, 2005, 77(17): 5 570-5 578.
[16]  Barton S J, Howe F A, Tomlins A M, et al. Comparison of in vivo 1H MRS of human brain tumours with 1H HR-MAS spectroscopy of intact biopsy samples in vitro[J]. Magma, 1999, 8(2): 121-128.
[17]  Griffin J L, Williams H J, Sang E, et al. Abnormal lipid profile of dystrophic cardiac tissue as demonstrated by one- and two-dimensional magic-angle spinning 1H NMR spectroscopy[J]. Magn Reson Med, 2001, 46(2): 249-255.
[18]  Waters N J, Holmes E, Waterfield C J, et al. NMR and pattern recognition studies on liver extracts and intact livers from rats treated with alpha-naphthylisothiocyanate[J]. Biochem Pharmacol, 2002, 64(1): 67-77.
[19]  Garrod S, Humpher E, Connor S C, et al. High-resolution 1H NMR and magic angle spinning NMR spectroscopic investigation of the biochemical effects of 2-bromoethanamine in intact renal and hepatic tissue\[J\]. Magn Reson Med, 2001, 45(5): 781-790.
[20]  Garrod S, Humpfer E, Spraul M, et al. High-resolution magic angle spinning 1H NMR spectroscopic studies on intact rat renal cortex and medulla[J]. Magn Reson Med, 1999, 41(6): 1 108-1 118.
[21]  Moka D, Vorreuther R, Schicha H, et al. Biochemical classification of kidney carcinoma biopsy samples using magic-angle-spinning 1H nuclear magnetic resonance spectroscopy\[J\]. J Pharm Biomed Anal, 1998, 17(1): 125-132.
[22]  Moka D, Vorreuther R, Schicha H, et al. Magic angle spinning proton nuclear magnetic resonance spectroscopic analysis of intact kidney tissue samples[J]. Anal Comm, 1997, 34: 107-109.
[23]  Tate A R, Foxall P J, Holmes E, et al. Distinction between normal and renal cell carcinoma kidney cortical biopsy samples using pattern recognition of 1H magic angle spinning (MAS) NMR spectra\[J\]. NMR Biomed, 2000, 13(2): 64-71.
[24]  Lucas L H, Wilson S F, Lunte C E, et al. Concentration profiling in rat tissue by high-resolution magic-angle spinning NMR spectroscopy: investigation of a model drug[J]. Anal Chem, 2005, 77(9): 2 978-2 984.
[25]  Chen Wen-xue(陈文学), Deng Feng(邓风), Yue Yong(岳勇). Applicationof NMR techniques to biological tissues(核磁共振技术在生物组织中的应用)[J]. Chinese J Magn Reson(波谱学杂志), 2004, 21(1): 127-139.
[26]  Cloarec O, Dumas M E, Craig A, et al. Statistical total correlation spectroscopy: an exploratory approach for latent biomarker identification from metabolic 1H NMR data sets[J]. Anal Chem, 2005, 77(5): 1 282-1 289.
[27]  Holmes E, Loo R L, Cloarec O, et al. Detection of urinary drug metabolite (xenometabolome) signatures in molecular epidemiology studies via statistical total correlation (NMR) spectroscopy\[J\]. Anal Chem, 2007, 79(7): 2 629-2 640.
[28]  Holmes E, Cloarec O, Nicholson J K. Probing latent biomarker signatures and in vivo pathway activity in experimental disease states via statistical total correlation spectroscopy (STOCSY) of biofluids: application to HgCl2 toxicity[J]. J Proteome Res, 2006, 5(6): 1 313-1 320.
[29]  Cloarec O, Campbell A, Tseng L H, et al. Virtual chromatographic resolution enhancement in cryoflow LC-NMR experiments via statistical total correlation spectroscopy[J]. Anal Chem, 2007, 79(9): 3 304-3 311.

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