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生防木霉菌Th2和T4对甜瓜根围土壤微生态的影响

, PP. 522-528

Keywords: 生防木霉菌,土壤微生物,T-RFLP分析,土壤微生物量碳

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Abstract:

利用平板计数、微生物量碳和末端标记限制性片段长度多态性分析(terminalrestrictionfragmentlengthpolymorphism,T-RFLP)相结合的方法研究了2株生防菌哈茨木霉Th2和T4对甜瓜根围土壤微生态的影响。结果表明,生防菌Th2和T4对根围土壤细菌有明显的促生作用,而对真菌和放线菌有明显的抑制作用。T-RFLP数据统计学分析表明,Th2和T4的引入可以增加土壤细菌的多样性。其中对开花期土壤细菌菌群影响最显著,Th2对TRF251和TRF513菌群有显著的促生作用,含量是对照的3.7倍和4.5倍,T4对TRF251和TRF513菌群含量也比对照高4倍和5.6倍,而2株生防菌对TRF60菌群均有显著的抑制作用,其含量分别为对照的51.6%和64.3%。在果熟期生防菌对土壤细菌菌群的影响减弱,表明土壤的缓冲功能发挥了作用。研究结果还表明,Th2和T4对土壤细菌菌群有着相似的促生和抑制作用,且不会破坏土壤微生态的稳定性。

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