全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

转Btcry1Ab基因水稻花粉对中华草蛉成虫产卵和寿命的影响

, PP. 225-228

Keywords: 转cry1Ab基因水稻,花粉,中华草蛉,产卵,寿命

Full-Text   Cite this paper   Add to My Lib

Abstract:

用ELISA法测定了转Bt基因水稻克螟稻1号(KMD1)和克螟稻2号(KMD2)花粉中Cry1Ab含量。将转Bt基因水稻和其亲本对照秀水11(XS11)花粉加入到草蛉人工干粉饲料中,评价了对中华草蛉ChrysoperlasinicaTjeder成虫产卵和寿命等参数的影响。结果显示,转Bt基因水稻"克螟稻"花粉中表达Cry1Ab杀虫蛋白,且表达量较高。分别用含5%KMD1、KMD2和XS11花粉的人工饲料饲喂中华草蛉初羽化成虫,发现两类Bt水稻花粉对该虫产卵前期、产卵量、产卵天数和成虫寿命等参数均无显著影响。人工饲料中水稻花粉的比例上升至20%和80%后,KMD1、KMD2和XS11花粉饲料处理中的草蛉均出现产卵前期延长、产卵量减少和产卵天数缩短的趋势,但在同一花粉比例下这些参数在3个花粉饲料处理间均无显著差异。结果表明,Bt水稻花粉中Cry1Ab杀虫蛋白对该草蛉成虫产卵和寿命等参数无显著的负面影响。

References

[1]  张少燕 谢宝瑜.转基因棉花Bt毒蛋白的表达及其生态学效应[J].昆虫知识,2002,39(5):328-335.
[2]  Jervis M A.Kidd N A C.Insect Natural Enemies.London:Chapman&Hall,1996,pp.375-394.
[3]  牟吉元 王念慈 范永贵.四种草蛉生活史和习性的研究[J].植物保护学报,1980,7(1):1-7.
[4]  更多...
[5]  杨集昆.草蛉的生活习性和常见种类[J].昆虫知识,1974,11(3):36-41.
[6]  赵敬钊.中华草蛉生物学及其种群消长的研究[J].昆虫天敌,1982,4(2):31-37.
[7]  Hilbeck A, Baumgartner M, Fried P M, et al. Effects of transgenic Bacillus thuringiensis corn-fed prey on mortality and development time of immature Chrysoperla carnea ( Neuroptera: Chrysopidae).Environmental Entomology, 1998, 27:480-487.
[8]  Hilbeck A, Moar W J, Pusztai-Carey M, et al. Toxicity of the Bacillus thuringiensis Cry1Ab toxin on the predator Chrysoperla carnea (Neuroptera: Chrysopidae) using diet incorporated bioassays. Environmental Entomology, 1998, 27: 1255- 1263.
[9]  Meier M S, Hilbeck A. Influence of transgenic Bacillus thuringiensis corn-fed prey on prey preference of immature Chrysoperla carnea (Neuroptera: Chrysopidae). Basic. Applied Entomology, 2001, 2(1): 35-44.
[10]  Head G, Brown C R, Groth M E, et al. Cry1Ab protein levels in phytophagous insects feeding on transgenic corn: implications for secondary exposure risk assessment. Entomologia Experimentalis et Applicata, 2001, 99:37-45.
[11]  Pilcher C D, Obrycki J J, Rice M E, et al. Preimaginal development, survival, and field abundance of insect predators on transgenic Bacillus thuringiensis corn. Environmental Entomology,1997, 26:446-454.
[12]  崔金杰 夏敬源.麦套夏播转Bt基因棉田主要害虫及其天敌的发生规律[J].棉花学报,:.
[13]  贾士荣 郭三堆 等.转基因棉花[M].北京:科学出版社,2001..
[14]  孙长贵 张青文 徐静 王因霞 刘俊丽.转Bt基因棉和转Bt+CpTI双价基因棉对棉田主要害虫及其天敌种群动态的影响[J].昆虫学报,2003,46(6):705-712.
[15]  董亮 万方浩 张桂芬 刘小京 李强.转Bt基因抗虫棉对中华草蛉发育及繁殖的影响研究[J].中国生态农业学报,2003,11(3):16-18.
[16]  Fearing P L, Brown D, Vlachos D, et al. Quantitative analysis of Cry1Ab expression in Bt maize plants, tissue, and silage and stability of expression over successive generations. Molecular Breeding, 1997, 3: 169 - 176.
[17]  周伟儒 刘志兰 邱式邦.用干粉饲料饲养中华草蛉成虫的研究[J].植物保护,1980,(5):2-3.
[18]  唐启义 冯明光.实用统计分析及其DPS数据处理系统[M].北京:科学出版社,2002.289-292.
[19]  李保平 万方浩 等.转基因抗虫植物对天敌昆虫的影响[J].中国生物防治,2002,18(3):97-105.
[20]  Lozzia G C, Furlanis C, Manachini B, et al. Effects of Bt corn on Rhopalosiphum padi ( Homoptera: Aphidae ) and on its predator Chrysoperla carnea Stephen ( Neuroptera: Chrysopidae). Bollettino di Zoologia Agrariae di Bachicoltura Seri. Ⅱ , 1998, 31: 153 - 164.
[21]  Romeis J,Dutton A, Bigler F. Bacillus thuringiensis toxin (Cry1Ab)has no direct effect on larvae of the green lacewing Chrysoperla carnea ( Stephens ) ( Neuroptera: Chrysopidae ). Journal of Insect Physiology, 2004, 50: 175 - 183.
[22]  Fujimoto H, Itoh K, Yamamoto M, et al. Insect resistant rice generated by introduction of a modified endotoxin gene of Bacillus thuringiensis. Bio/Technology, 1993, 11:1151-1155.
[23]  Wünn J, Kloti A, Burkhardt P K, et al. Transgenic indica rice breeding line IR58 expressing a synthetic cryIA(b) gene from Bacillus thuringiensis provides effective insect pest control. Bio/Technology, 1996, 14 (2): 171-176.
[24]  Riazuddin S. Transformation of indica rice with Bt pesticidal genes.In: Rice Genetics Ⅲ: Proceeding of the Third International Rice Genetics Symposium, 16- 20, October, 1995, Manila (Philippines): IRRI, 1996, pp. 730-734.
[25]  Ghareyazie B, Alinia F, Menguito C A, et al. Enhanced resistance to two stem borers in an aromatic rice containing a synthetic cryIA(b) gene. Molecular Breeding, 1997, 3: 401 -414.
[26]  Nayak P, Basu D, Das S, et al. Transgenic elite indica rice plants expressing cryIA (c) endotoxin of Bacillus thuringiensis are resistant against yellow stem borer (Scirpophaga incertulas). Proceedings of the National Academy of Sciences, USA, 1997, 94: 2111 - 2116.
[27]  Wu C, Fan Y, Zhang C, et al. Transgenic fertile japonica rice plants expressing a modified cryIA (b) gene resistant to yellow stem borer. Plant Cell Reporter, 1997, 17: 129-132.
[28]  Datta K, Vasquez A, Tu J, et al. Constitutive and tissue-specific differential expression of the cryIA (b) in transgenic rice plants conferring resistance to rice insect pests. Theoretical and Applied Genetics. , 1998, 97:31 -36.
[29]  舒庆尧 叶恭银.转基因水稻“克螟稻”选育[J].浙江农业大学学报,:.
[30]  Cheng X Y, Sardana R, Kaplan H, et al. Agrobacterium-transformed rice plants expressing synthetic cryIA(b) and cryIA(c) genes are highly toxic to yellow stem borer and striped stem borer. Proceedings of the National Academy of Sciences, USA, 1998, 95:2767 - 2772.
[31]  吴刚 Altos.,L.cry1Ab基因在转基因“克螟稻”后代中的遗传稳定性及表达[J].农业生物技术学报,:.
[32]  Shu Q Y, Ye Q Y, Cui H R, et al. Transgenic rice plants with a synthetic cry1Ab gene from Bacillus thuringiensis were highly resistant to eight lepidopteran rice pest species. Molecular Breeding,2000, 6 : 433 - 439.
[33]  Masood E. G8 leaders seek study on effects of biotech. Nature,1999, 399:717.
[34]  钱迎倩 田彦.转基因植物的生态风险评价[J].植物生态学报,:.
[35]  Losey J E, Rayor L S, Carter M E. Transgenic pollen harms monarch larvae. Nature, 1999, 399:214.
[36]  Saegusa A. Japan tightens rules on GM crops to protect the environment. Nature, 1999, 399:719.
[37]  Poppy G. GM crops: environmental risks and non-target effects.Trends in Plant Science, 2000, 5 ( 1 ): 4 - 6.
[38]  张永军 吴孔明 彭于发 郭予元.转基因植物的生态风险[J].生态学报,:.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133