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转葡聚糖酶和防御素基因的番茄植株及其对番茄灰霉病的抗性

, PP. 357-362

Keywords: 转基因番茄,葡聚糖酶基因,苜蓿防御素基因,灰霉病

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Abstract:

用根癌农杆菌Agrobacteriumtumesfaciens介导法,将烟草β-1,3-葡聚糖酶Glucanase基因、苜蓿防御素alfAFP基因及二者双价基因导入番茄Micro-Tom中,以期获得抗灰霉病的转基因株系,并比较验证双价基因是否具有协同作用。绿色荧光蛋白基因(GFP)活性检测、PCR和Southern杂交检测表明,外源基因分别以1~4个拷贝整合到番茄基因组中。对T1代植株进行接种鉴定表明,转基因植株对灰霉病侵染的抵抗能力较对照明显增强,各株系的抗病能力与外源基因的表达相关,且葡聚糖酶基因和防御素基因对抗灰霉菌表现一定的协同作用。T2株系田间抗病鉴定结果表明,转基因株发病程度不同,病情指数在19~28之间,抗病性均高于对照。所得到的稳定表达的株系可作为抗病育种材料进一步研究。

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