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类黄酮在草甘膦诱导的苦荞膜脂过氧化中的作用

, PP. 412-416

Keywords: 草甘膦,类黄酮,膜脂过氧化,自由基,苦荞

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Abstract:

研究了草甘膦对苦荞类黄酮次生代谢的影响及类黄酮与草甘膦作用下膜脂过氧化伤害的关系,以探讨植物类黄酮代谢的意义及在草甘膦伤害中的作用机制。结果表明,分别用浓度为0.1、0.3、1mmol/L的草甘膦处理苦荞幼苗,苦荞类黄酮代谢受到明显抑制,处理3天时类黄酮含量比对照分别下降58.1%、65.8%和76.5%。草甘膦处理导致苦荞膜脂过氧化加剧,0.1mmol/L草甘膦处理使苦荞相对电导率增加275.4%、丙二醛(MDA)含量增加134.1%、超氧自由基O-2.产生速率增加121.7%,且随草甘膦浓度升高而增加幅度加大,说明草甘膦伤害与膜脂过氧化程度有关。0.3mmol/L草甘膦处理后再用0.1mmol/L类黄酮物质芦丁处理,电解质外渗下降34.2%,MDA含量下降51.1%,O-2.产生下降33.9%,明显减轻了草甘膦的伤害,这说明草甘膦作用下类黄酮含量的下降与草甘膦对苦荞组织伤害有一定的关系。

References

[1]  Zhang H Y,Chen D Z.Theoretical elucidation on activity differences of ten flavonoid antioxidants.Acta Biochemica et Biophysica Sinica,2000,32 (4):317 -321
[2]  Waldecker M A,Wyse D L.Chemical and physical effects of the accumulation of glyphosate in common milk weed (Asclepiax syriaca) root buds.Weed Science,1985,33 (5):605-611
[3]  Papanikou E,Brotherton J E,Widholm J M.Length of time in tissue culture can affect the selected glyphosate resistance mechanism.Planta,2004,218:589-598
[4]  刘支前.草甘膦防治菟丝子的机理研究.植物保护学报,1994,21(4):369-372
[5]  陈虎保,朱惠香,陈国海.草甘膦的作用机理及部位.林业科技通讯,1997,1:23-25
[6]  唐宇,赵钢.荞麦中黄酮含量的研究.四川农业大学学报,2001,19(4):352-354
[7]  朱友春,田世龙,王东晖.比色法测定苦荞中黄酮含量的方法改进.甘肃科技,2003,19(2):13-14
[8]  赵世杰,史国安,董新纯.植物生理学实验指导.北京:中国农业科学技术出版社,2002
[9]  王爱国,罗广华.植物的超氧化物自由基与羟胺反应的定量关系.植物生理学通讯,1990,6(1):55-57
[10]  Eschenburg S,Healy M L,Melanie A.How the mutation glycine 96 to alanine confers glyphosate insensitivity to 5 -enolpyruvyl shikimate-3-phosphate synthase from Escherichia coli.Planta,2002,216:129-135
[11]  Nadler-Hassar T,Goldshmidt A,Rubin B.Glyphosate inhibits the translocation of green fluorescent protein and sucrose from a transgenic tobacco host to Cuscuta campestris Yunk.Planta,2004,219:790-796
[12]  Wink M.Evolution of secondary metabolites from an ecological and molecular phylogenetic perspective.Phytochemistry,2003,64 (1):3 -19
[13]  Schnitzler J P,Jungblut T P,Feicht C.UV-B induction of flavonoid biosynthesis in scots pine (Pinus sylvestris L.) seedlings.Trees,1997,11:162-168
[14]  Teramura A H,Ziska L H,Sztein A E.Changes in growth and photosynthetic capacity of rice with increased UV-B radiation.Physiologia Plantarum,1991,83(3):373 -380
[15]  Yokozawa T,Dong E,Liu Z W,et al.Antioxidantive activity of flavones and flavonols in vitro.Phytotherapy Research,1997,11 (6):446-449
[16]  张美莉,胡小松.荞麦生物活性物质及其功能研究进展.杂粮作物,2004,24(1):26-29
[17]  Packer L,Rim B G.Antioxidant activity and biologic properties of a procyanidin-rich extract from pine (Pinus maritimea) bark,pycnogenol.Free Radical Biology and Medicine,1999,27(5 -6):704 -724
[18]  Schubert S Y,Lansky E P,Neeman Ⅰ.Antioxidant and eicosanoid enzyme inhibition properties of pomegranate seed oil and fermented juice flavonoids.Journal of Ethnopharmacology,1999,66 (1):11-17
[19]  Kameoka S,Leavitt P,Chang C S,et al.Expression of antioxidant proteins in human in testinal Caco-2 cells treated with dietary flavonoids.Cancer Letters,1999,146(2):161-167
[20]  Wang H,Joseph J A.Structure-activity relationships of querceein in an agonizing hydrogen peroxidr-induced calcium deregulation in PC1 2 cells.Free Radical Biology and Medicine,1999,27 (5 -6):683-694

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