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四川米易赛葵上粉虱传双生病毒的分子鉴定及复合侵染检测

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Keywords: 赛葵,粉虱传双生病毒,卫星DNAβ,滚环扩增,复合侵染

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Abstract:

为了明确四川米易赛葵上的双生病毒种类及复合侵染情况,基于滚环扩增PCR(RCA-PCR)技术,利用双生病毒DNA-A、DNA-B及卫星DNA的通用引物对四川米易表现黄脉症状的12个赛葵样品进行了检测,并对其序列进行分析。双生病毒的检出率高达92%;共检出云南赛葵黄脉病毒(MalvastrumyellowveinYunnanvirus,MYVYNV)、赛葵黄脉病毒(Malvastrumyellowveinvirus,MYVV)及中国番茄黄化曲叶病毒(TomatoyellowleafcurlChinavirus,TYLCCNV)等3种双生病毒,复合侵染率高达67%;11个阳性样品中共检测到两类卫星DNAβ分子,分别与MYVYNV伴随的DNAβ(MYVYNB-,98.4%~98.7%)和MYVV伴随的DNAβ(MYVB-,98.5%~98.7%)的序列相似性最高;未扩增到DNA-B及DNAα分子。表明病毒DNA-A与卫星DNAβ以病害复合体形式存在,且DNAβ可伴随异源辅助病毒。

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