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水稻条纹病毒胁迫对灰飞虱mucin基因表达量的影响

, PP. 289-293

Keywords: 灰飞虱,mucin基因,RSV胁迫,实时荧光定量PCR

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Abstract:

为分析灰飞虱mucin基因在水稻条纹病毒(Ricestripevirus,RSV)侵染过程中的作用,采用荧光定量PCR的方法分析了RSV胁迫条件下灰飞虱mucin基因mRNA表达量的变化。饲喂RSV病叶1天和2天时灰飞虱mucin基因的表达量没有明显变化,而在3天和7天时灰飞虱mucin基因的表达量增加了3.60倍和1.97倍。饲喂健康水稻叶片1、2、3和7天后,灰飞虱mucin基因的表达量分别为饲喂前的1.07、1.12、0.78和0.34倍,而饲喂病叶1、2、3和7天后,mucin基因的表达量分别为饲喂前的1.15、1.19、3.19和1.01倍。结果表明,病毒胁迫使灰飞虱体内mucin基因的表达量增加,暗示mucin基因在RSV与灰飞虱互作过程中起着重要作用。

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