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SYBRGreenⅠ实时定量RT-PCR技术在甜樱桃病毒定量分析中的应用

, PP. 497-502

Keywords: 李属坏死环斑病毒,李矮缩病毒,樱桃病毒A,樱桃小果病毒-2,实时定量RT-PCR

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Abstract:

为了探讨SYBRGreenⅠ实时定量RT-PCR技术在甜樱桃病毒粒子定量分析中的应用前景,以复合感染李属坏死环斑病毒(Prunusnecroticringspotvirus,PNRSV)、李矮缩病毒(Prunedwarfvirus,PDV)、樱桃病毒A(CherryvirusA,CVA)、樱桃小果病毒-2(Littlecherryvirus-2,LChV-2)的甜樱桃"红灯"Prunusaviumcv.RedLamp植株为研究对象,采用相对定量方法,分析各病毒的外壳蛋白基因的表达,用以指示病毒的增殖量。在花、幼叶、功能叶、衰老叶中均能检测到4个基因,但各基因表达量在各器官中存在差异。PNRSV-CP与CVA-CP表达模式相似,功能叶中明显高于其它器官,衰老叶中急剧降低。PDV-CP与LChV2-CP表达模式类似,幼叶中的表达量较高,功能叶片中较低。PNRSV-CP在花、功能叶中的表达显著高于其它3个病毒基因。LChV2-CP在各器官中的表达量均低于其余3个基因。该方法适用于植物组织内多种甜樱桃病毒增殖量的分析。

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