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苹果花叶病毒的RT-PCR检测及其在我国苹果产区的分布

, PP. 443-448

Keywords: 苹果花叶病毒,RNA提取,RT-PCR,分布

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Abstract:

为了开发苹果花叶病毒(Applemosaicvirus,ApMV)田间样本RT-PCR检测方法和揭示我国ApMV的发生情况,以田间染病苹果组织为材料,对ApMVRT-PCR检测体系中总RNA提取方法、反应体系及程序进行了选择和优化,并利用优化的检测方法对我国苹果主产区13个省的23个市(县)ApMV发生情况进行了检测。以RNA提取改良法提取的田间样本总RNA为模板,RT-PCR优化体系的灵敏度达到能够检测15μg田间样本组织中的ApMV,且在果树整个生长发育时期能够检测1年生枝条树皮组织的带毒情况;ApMV在我国普遍发生,13个省的23个市(县)均检测到了ApMV,采集的327个样本带毒率为80.1%,其中未显花叶症状样本205个,ApMV阳性率为68.3%;在23个地区中,只有河北张家口、河南三门峡和陕西白水样本带毒率低于50%。

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