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宁夏固原致病疫霉群体结构特征

, PP. 418-424

Keywords: 交配型,甲霜灵抗性,线粒体DNA单倍型,简单重复序列,扩增片段长度多态性

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Abstract:

为揭示宁夏固原致病疫霉群体遗传结构,用对峙培养法和菌落直径法测定致病疫霉的交配型和甲霜灵抗性,利用限制性片段长度多态性聚合酶链反应(PCR-RFLP)技术检测致病疫霉线粒体DNA单倍型,并采用简单重复序列(SSR)和扩增片段长度多态性(AFLP)技术确定致病疫霉群体的基因型。在94株供试菌株中,发现了1株A1交配型、86株A2交配型及7株自育型菌株,全部为甲霜灵抗性菌株。菌株的线粒体单倍型均为Ⅰa型,共鉴定出2种SSR基因型,Ⅰ型占绝对优势。AFLP聚类分析显示,在相似系数0.68时分成α和β两组,宁夏固原菌株全部聚在β组。研究表明,宁夏固原致病疫霉群体结构比较单一、遗传多样性水平较低。

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