全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

黄淮麦区小麦全蚀病菌群体的遗传多样性分析

, PP. 495-501

Keywords: 小麦全蚀病菌,SSR标记,群体遗传多样性,基因流

Full-Text   Cite this paper   Add to My Lib

Abstract:

为了解小麦全蚀病病菌的群体组成和遗传多样性,采用8对多态性较好的微卫星标记,对我国黄淮麦区的116个小麦全蚀病菌株进行了分析。8个SSR标记的平均等位基因数为4.25个,多态性信息含量的平均值为0.66。供试菌株的平均有效等位基因数和基因遗传多样性指数分别为1.46和0.27,漯河群体的遗传多样性水平最高,周口群体最低。不同群体间遗传距离均较小,为0.0199~0.1153,其中徐州和周口群体间的遗传距离相对较大,而周口和驻马店群体间的遗传距离相对较小。分子方差分析结果显示,小麦全蚀病菌群体间和群体内均存在着一定的遗传分化,群体间的遗传变异占总变异的10%,群体内遗传变异占90%。6个群体间的基因流为3.5。根据SSR多态性,对来源不同菌株的UPGMA聚类分析结果显示,小麦全蚀病菌群体结构与地理来源有一定的关系。

References

[1]  Bateman G L, Ward E, Hornby D, et al. Comparisons of isolates of the take all fungus, Gaeumannomyces graminis var.tritici, from different cereal sequences using DNA probes and non molecular methods. Soil Biology and Biochemistry, 1997, 29(8): 1225-1232
[2]  张秋娥, 曹克强, 胡同乐, 等. 河北省小麦全蚀病菌变种类型鉴定. 植物保护, 2008, 34(4): 18-21
[3]  Fouly H M, Wilkinson H T. Detection of Gaeumannomyces graminis varieties using polymerase chain reaction with variety specific primers. Plant Disease, 2000, 84(9): 947-951
[4]  王宁, 冯彦霞, 杜文珍, 等. 小麦全蚀病菌致病力测定及品种抗病性研究. 植物遗传资源学报, 2012, 13(3): 478-483
[5]  Sanguinetti C J, Dias N E, Simpson A J. Rapid silver staining and recovery of PCR products separated on polyacrylamide gels. Biotechniques, 1994, 17(5): 914-921
[6]  王东东, 李良秋, 马连营, 等. 大型真菌中SSR分子标记的开发与应用进展. 微生物学通报, 2013, 40(4): 646-654
[7]  Wright S. Evolution in Mendelian populations. Genetics, 1931, 16(2): 97-99
[8]  Cook R J. Take all of wheat. Physiological and Molecular Plant Pathology, 2003, 62(2): 73-86
[9]  Anderson J A, Churchill G A, Autrique J E, et al. Optimizing parental selection for genetic linkage maps. Genome, 1993, 36(1): 181-186
[10]  Lebreton L, Lucas P, Dugas F, et al. Changes in population structure of the soilborne fungus Gaeumannomyces graminis var.tritici during continuous wheat cropping. Environmental Microbiology, 2004, 6(11): 1174-1185
[11]  Augustin C, Ulrich K, Ward E, et al. RAPD based inter and intravarietal classification of fungi of the Gaeumannomyces Phialophora complex. Journal of Phytopathology, 1999, 147(2): 109-117
[12]  Bryan G T, Labourdette E, Melton R E, et al. DNA polymorphism and host range in the take all fungus, Gaeumannomyces graminis. Mycological Research, 1999, 103(3): 319-327
[13]  Bryan G T, Daniels M J, Osbourn A E. Comparison of fungi within the Gaeumannomyces Phialophora complex by analysis of ribosomal DNA sequences. Applied and Environmental Microbiology, 1995, 61(2): 681-689
[14]  Freeman J, Ward E, Gutteridge R J, et al. Methods for studying population structure, including sensitivity to the fungicide silthiofam, of the cereal take all fungus, Gaeumannomyces graminis var.tritici. Plant Pathology, 2005, 54(5): 686-698
[15]  Sadeghi L, Alizadeh A, Safaie N, et al. Genetic diversity of Gaeumannomyces graminis var.tritici populations using RAPD and ERIC markers. Journal of Plant Pathology & Microbiology, 2012, 3(7): 143-147
[16]  Rassmann K,Schlotterer C,Tautz D. Isolation of simple sequence loci for use in polymerase chain reaction based DNA fingerprinting. Electrophoresis, 1991, 12(2/3): 113-118
[17]  Tóth G, Gáspári Z, Jurka J. Microsatellites in different eukaryotic genomes: survey and analysis. Genome Research, 2000, 10(7): 967-981
[18]  Kantety R V, La Rota M, Matthews D E, et al. Data mining for simple sequence repeats in expressed sequence tags from barley, maize, rice, sorghum and wheat. Plant Molecular Biology, 2002, 48(5/6): 501-510
[19]  Sirjusingh C, Kohn L M. Characterization of microsatellites in the fungal plant pathogen, Sclerotinia sclerotiorum. Molecular Ecology Notes, 2005, 1(4): 267-269
[20]  Yeh F C, Yang R, Boyle T. Popgene version 1.31. Microsoft Window Based Freeware for Population Genetic Analysis, 1999
[21]  Peakall R, Smouse P E. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes, 2006, 6(1): 288-295
[22]  Nei M. Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of the United States America, 1973, 70(12): 3321-3323
[23]  更多...
[24]  孙海燕, 李琦,杜文珍, 等. 不同杀菌剂拌种防治小麦全蚀病研究. 植物保护, 2012, 38(3): 155-158
[25]  徐飞, 杨共强, 何文兰, 等. 不同小麦品种(系)对全蚀病的抗性鉴定与评价. 植物保护, 2013, 39(2): 143-146
[26]  Weber Z, Irzykowska L, Bocianowski J. Analysis of mycelial growth rates and RAPD PCR profiles in a population of Gaeumannomyces graminis var.tritici originating from wheat plants grown from fungicide treated seed. Journal of Phytopathology, 2005, 153(6): 318-324
[27]  段晓莉, Witold L, Malgorzata J, 等. 利用微卫星标记分析核盘菌遗传多样性. 中国油料作物学报, 2012, 34(3): 294-299
[28]  许敏青, 王坤, 孟庆芳, 等. 我国部分地区小麦叶锈菌遗传多样性的SSR分析. 农业生物技术学报, 2013, 21(1):89-96

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133