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转cry1Ab/cry2Aj玉米对亚洲玉米螟的抗性评价

, PP. 339-344

Keywords: 转cry1Ab/cry2Aj玉米,亚洲玉米螟,抗虫性

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Abstract:

为探讨转cry1Ab/cry2Aj玉米对亚洲玉米螟Ostriniafurnacalis(Guenée)的抗性,采用室内生测和田间人工接虫鉴定方法评价了6个转cry1Ab/cry2Aj玉米品系对亚洲玉米螟的杀虫效果,并利用酶联免疫吸附法(ELISA)测定了Cry1Ab蛋白在各品系主要组织的表达水平。结果显示,亚洲玉米螟幼虫取食转基因玉米各品系雄穗、苞叶、花丝和雌穗48h后死亡率在90%左右,取食心叶的死亡率在70%左右,96h后大部分幼虫死亡,而取食非转基因玉米的死亡率为3.3%~8.7%;春播和夏播田转基因玉米叶片、茎秆、雌穗基本没有被害,而对照玉米叶片、茎秆、雌穗被害严重,春播和夏播玉米平均每株分别有2.9、2.3头幼虫和蛹,2.8、3.4个蛀孔,隧道长度为7.5、14.2cm,雌穗被害级别为5.3和5.1;6个转基因玉米品系中Cry1Ab蛋白在心叶、雄穗、花丝、苞叶和雌穗中稳定表达。综合评价认为,转基因玉米各品系在整个生育期内对亚洲玉米螟有很好的抗性,其中N50品系抗虫效果最佳,可以作为抗虫转多基因玉米育种的备选材料。

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