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一氧化氮对果蔬采后灰霉菌生长发育及致病性的影响

, PP. 243-248

Keywords: 一氧化氮,采后病害,灰霉菌,活性氧

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Abstract:

为探讨NO的抑菌效果,以硝普钠(sodiumnitroprusside,SNP)为NO供体,进行番茄活体接种试验,研究外源NO处理对采后灰霉菌Botrytiscinerea生长发育及致病性的影响,并通过胞内抗氧化相关酶活性和丙二醛(MDA)含量以及活性氧水平的检测,探讨其作用机制。结果显示,SNP的最低抑菌浓度为4mmol/L;SNP处理灰霉菌5?h后,与对照组相比,孢子萌发率、芽管长度分别降低56%和25?μm,菌丝扩展速度下降31%;胞内过氧化氢酶、过氧化物酶、抗坏血酸过氧化物酶、谷胱甘肽还原酶活性分别下降30%、42%、38%、40%,脂氧合酶活性和MDA含量分别提高48%和67%;SNP处理灰霉菌后接种番茄6?d,发病率下降22%,病斑直径减小12?mm;胞内活性氧积累约为对照组的3倍。表明NO能够显著抑制灰霉菌的生长发育,其抑菌机制是通过诱导灰霉菌胞内活性氧积累引起氧化损伤。

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