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禾谷缢管蚜水通道蛋白基因RpAQP1的克隆、分子特性和表达分析

, PP. 657-664

Keywords: 禾谷缢管蚜,水通道蛋白,基因克隆,分子特性,表达分析

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Abstract:

为探究禾谷缢管蚜Rhopalosiphumpadi(Linnaeus)水通道蛋白基因RpAQP1的序列特征及其在不同龄期的表达,利用RT-PCR和RACE技术克隆了RpAQP1基因的cDNA全序列,采用生物信息学软件分析了RpAQP1编码蛋白的特性,利用qRT-PCR分析了RpAQP1在不同龄期的表达量。结果显示:禾谷缢管蚜水通道蛋白基因RpAQP1的cDNA全长为1216bp,其750bp的开放阅读框编码250个氨基酸,蛋白分子量为27.36kD。RpAQP1属于水通道蛋白亚家族DRIP(果蝇内嵌蛋白Drosophilaintegralprotein)的一员,具有6个跨膜区,2个保守的NPA结构单元和1个压缩区域Ar/R;qRT-PCR结果显示,RpAQP1在整个发育历期均有表达,其在2龄若蚜中表达水平最高,是成蚜表达量的1.432倍;在4龄若蚜中表达量最低,为成蚜表达量的0.444倍,显著低于其它龄期,而其余各龄期RpAQP1表达量无显著差异。

References

[1]  Douglas A. Phloem-sap feeding by animals: problems and solutions. Journal of Experimental Botany, 2006, 57 (4):747-754
[2]  Shakesby A J, Wallace I S, Isaacs H V, et al. A water-specificaquaporin involved in aphid osmoregulation. Insect Biochemistry and Molecular Biology, 2009, 39(1): 1-10
[3]  Mathew L G, Campbell E M, Yool A J, et al. Identificationand characterization of functional aquaporin water channel protein from alimentary tract of whitefly, Bemisiatabaci. Insect Biochemistry and Molecular Biology, 2011, 41(3): 178-190
[4]  Campbell E M, Ball A, Hoppler S, et al. Invertebrate aquaporins: a review. Journal of Comparative Physiology B, 2008, 178(8): 935-955
[5]  Ishibashi K, Hara S, Kondo S. Aquaporin water channels inmammals. Clinical and Experimental Nephrology, 2009, 13(2): 107-117
[6]  Benga G. Water channel proteins (later called aquaporins) andrelatives: past, present, and future. IUBMB Life, 2009, 61(2): 112-133
[7]  Rao Y, Jan L Y, Jan Y N. Similarity of the product of the Dro-sophila neurogenic gene big brain to transmembrane channel proteins. Nature, 1990, 345(6271): 163-167
[8]  Beuron F, Le Cahérec F, Guillam M T, et al. Structural analy-sis of a MIP family protein from the digestive tract of Cicadellaviridis. Journal of Biological Chemistry, 1995, 270 (29):17414-17422
[9]  Le Cahérec F, Deschamps S, Delamarche C, et al. Molecularcloning and characterization of an insectaquaporin functionalcomparison with aquaporin 1. European Journal of Biochemistry, 1996, 241(3): 707-715
[10]  Spring J H, Robichaux S R, Hamlin J A. The role of aquaporins in excretion in insects. Journal of Experimental Biology,2009, 212(Pt3): 358-362
[11]  King L S, Kozono D, Agre P. From structure to disease: theevolving tale of aquaporin biology. Nature Reviews MolecularCell Biology, 2004, 5(9): 687-698
[12]  Rojek A, Praetorius J, Fr?kiaer J, et al. A current view of themammalian aquaglyceroporins. Annual Review of Physiology,2008, 70: 301-327
[13]  Izumi Y, Sonoda S, Yoshida H, et al. Role of membranetransport of water and glycerol in the freeze tolerance of therice stem borer, ChilosuppressalisWalker (Lepidoptera:Pyralidae). Journal of Insect Physiology, 2006, 52 (2):215-220
[14]  Cohen E. Water homeostasis and osmoregulation as targets inthe control of insect pests. Advances in Insect Physiology,2013, 44: 1-61
[15]  Detmers F J, de Groot B L, Müller E M, et al. Quaternaryammonium compounds as water channel blockers. Specificity,potency, and site of action. Journal of Biological Chemistry,2006, 281(20): 14207-14214
[16]  Liu K, Tsujimoto H, Cha S J, et al. Aquaporin water channelAgAQP1 in the malaria vector mosquito Anopheles gambiaeduring feeding and humidity adaptation. Proceedings of theNational Academy of Sciences of the United States of America,2011, 108(15): 6062-6066
[17]  Migliat E, Meurice N, DuBois P, et al. Inhibition of aquaporin-1and aquaporin-4 water permeability by a derivative of the loop diuretic bumetanide acting at an internal poreoccludingbindingsite. Molecular Pharmacology, 2009, 76(1): 105-112
[18]  Liu Aizhi, Li Shixun, Ru Taoqin, et al. Susceptibility and selective toxicity of some insecticides toSchizaphisgraminumFabricius andRhopalosiphimpadiLinn. Pesticide Science and Administration, 2001(S): 12-13(in Chinese) [刘爱芝, 李世功, 茹桃勤, 等. 杀虫剂对2种麦蚜的敏感性和选择性测定. 农药科学与管理, 2001(S): 12-13]
[19]  Zhang Jun, Liu Jing, Wang Genxuan, et al. Effect of elevated atmospheric CO2 concentration on Rhopalsiphumpadi population under different soil water levels. Chinese Jojurnalof Applied Ecology, 2001, 12(2):253-256 (in Chinese) [张钧, 刘敬, 王根轩, 等. 不同土壤水分条件下CO2 浓度对禾谷缢管蚜种群的影响. 应用生态学报, 2001, 12(2):253-256]
[20]  Lu Yanhui, Yang Ting, GaoXiwu. Establishment of baseline susceptibility data to various insecticides for aphids Rhopalosiphumpadi(Linnaeus) and Sitobionavenae (Fabricius) (Homoptera: Aphididae) by the method of residual film in glass tube. ActaEntomologicaSinica,2009, 52 (1):52-58(in Chinese) [鲁艳辉, 杨婷, 高希武. 禾谷缢管蚜和麦长管蚜玻璃管药膜法敏感毒力基线的建立.昆虫学报, 2009, 52 (1):52-58]
[21]  更多...
[22]  Hong Bo, Guan Xiaoqing, Chi Yongwei, et al. Bioassay of the sensitivity of wheat aphids to several insecticides in Ningxia. Journal of Ningxia Agricultural College, 2004, 25(3): 17-20(in Chinese) [洪波, 关晓庆, 迟永伟, 等. 宁夏地区麦蚜对几种杀虫剂的敏感性测定. 宁夏农学院学报(农业科学版), 2004, 25(3): 17-20]
[23]  Chen M H, Han Z J. Cloning and sequence analysis of 2 different acetylcholinesterase genes inRhopalosiphumpadi andSitobionavenae. Genome, 2006, 49(3): 239-243]
[24]  Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2 ? ΔΔCTmethod. Methods, 2001, 25(4): 402-408
[25]  Wang Xun. Cloning and experession of csaqpl genes inrice stem borer,ChilosuppressalisWalker(Lepidoptera:Pyralidae).Master Thesis.Yangzhou: Yangzhou University,2011:54-55 (in Chinese) [王勋. 水稻二化螟CsAQP1基因的克隆与表达. 硕士毕业论文. 扬州: 扬州大学, 2011: 54-55]
[26]  Liu Haiyuan, Shu Benshui, Jiang Chunlai, et al. Molecular cloning, characterization and expression analysis of aquaporin 1 (AQP1) gene in Spodopteralitura (Lepidoptera: Noctuidae).ActaEntomologicaSinica, 2013, 56(4): 339-349(in Chinese) [刘海远, 舒本水, 姜春来, 等. 斜纹夜蛾水通道蛋白1(AQP1)基因的克隆、分子特性和表达分析. 昆虫学报,2013, 56(4): 339-349]

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