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低温胁迫对异色瓢虫成虫存活及体内几种酶活力的影响

, PP. 513-518

Keywords: 异色瓢虫,低温胁迫,酶活力,低温伤害

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Abstract:

为探讨低温胁迫对异色瓢虫存活的影响以及冷伤害机制,采用温度和时间双因子处理测定了低温暴露中异色瓢虫成虫的存活率及其体内4种酶活性变化.结果表明:过冷却点以上的低温(-5、-7℃)就能导致异色瓢虫成虫大量死亡,随着温度的降低和暴露时间的延长,成虫存活率显著降低;暴露在-1、-3、-5和-7℃下的半致死时间LT50分别为8.30、6.68、3.54和1.24d,且随着温度的降低,LT50显著降低.异色瓢虫成虫体内过氧化氢酶和过氧化物酶活性随着温度的降低及暴露时间的延长而明显升高,在-10℃和-5℃下暴露10d时活性达到最高,分别为679.73U/g和396.06mg/mL;而乳酸脱氢酶和Na+、K+-ATP酶活性随着低温胁迫时间的延长而降低.表明低温下异色瓢虫成虫体内细胞保护酶系统的防御能力趋强而有利于增强低温抵抗力,但会影响其体内代谢产物及离子运输,长时间的低温胁迫甚至会引起代谢紊乱.

References

[1]  Kang Le. Insect-plant correlation under environment stress. Chinese Journal of Ecology, 1995, 14(5): 51-57 (in Chinese) [康乐. 环境胁迫下的昆虫-植物的相互关系. 生态学杂志, 1995, 14(5): 51-57]
[2]  Sinclair B J, Vernon P, Klok C J, et al. Insects at low temperatures: an ecological perspective. Trends in Ecology and Evolution, 2003, 18(5): 257-262
[3]  Bale J S, Worland M R, Block W. Effect of summer frost exposures on the cold tolerance strategy of a sub-Antarctic beetle. Journal of Insect Physiology, 2001, 47(10): 1161-1167
[4]  ?lachta M, Berková P, Vambera J, et al. Physiology of the cold acclimation in non-diapausing adults ofPyrrhocoris apterus (Heteroptera). European Journal of Entomology, 2002, 99(2): 181-187
[5]  Ko?tál V, Vambera J, Bastl J. On the nature of pre-freeze mortality in insects: water balance, ion homeostasis and energy charge in the adults of Pyrrhocoris apterus. The Journal of Experimental Biology, 2004, 207(9): 1509-1521
[6]  Ko?tál V, Yanagimoto M, Bastl J. Chilling-injury and disturbance of ion homeostasis in the coxal muscle of the tropical cockroach (Nauphoeta cinerea). Comparative Biochemistry and Physiology, Part B, Biochemistry & Molecular Biology, 2006, 143(2): 171-179
[7]  Chen B, Kang L. Cold hardiness and supercooling capacity in the pea leafminer Liriomyza huidobrensis. Cryo Letters, 2002, 23(3): 173-182
[8]  Colinet H, Renault D, Hance T, et al. The impact of fluctuating thermal regimes on the survival of a cold-exposed parasitic wasp, Aphidius colemani. Physiological Entomology, 2006, 31(3): 234-240
[9]  Jing Xiaohong, Kang Le. Research progress in insect cold hardines. Acta Ecologica Sinica, 2002, 22(12): 2202-2207 (in Chinese)[景晓红, 康乐. 昆虫耐寒性研究. 生态学报, 2002, 22(12): 2202-2207]
[10]  S?mme L. The physiology of cold hardiness in terrestrial arthropods. European Journal of Entomology, 1999, 96(1): 1-10
[11]  Renault D, Salin C, Vannier G, et al. Survival at low temperatures in insects: what is the ecological significance of the supercooling point? Cryo Letters, 2002, 23(4): 217-228
[12]  Hart A J, Bale J S, Tullet A G, et al. Effects of temperature on the establishment potential of the predatory mite Amblyseius californicus (Acari: Phytoseiidae) in the UK. Journal of Insect Physiology, 2002, 48(6): 593-599
[13]  Hatherly I S, Bale J S, Walters K F A, et al. Thermal biology of Typhlodromus montdorensis: implications for its introduction as a glasshouse biological control agent in the UK. Entomologia Experimentalis et Applicata, 2004, 111(2): 97-109
[14]  Koch R L. The multicolored Asian lady beetle, Harmonia axy-ridis: a review of its biology, uses in biological control, and non-target impacts. Journal of Insect Science, 2003, 3: 32#
[15]  Wang Su, Zhang Runzhi, Zhangfan. Research progress on biology and ecology of Harmonia axyridis Pallas (Coleoptera: Coccinellidae). Chinese Journal of Applied Ecology, 2007, 18(9): 2117-2126 (in Chinese) [王?, 张润志, 张帆. 异色瓢虫生物生态学研究进展. 应用生态学报, 2007, 18(9): 2117-2126]
[16]  Sakurai H, Kawai T, Takeda S. Physiological changes related to diapause of the lady beetle, Harmonia axydis (Coleoptera: Coccinellidae). Applied Entomology and Zoology, 1992, 27(4): 479-487
[17]  Zhao Jing, Yu Lingyuan, Li Min, et al. Seasonal variation in cold tolerance of the multicolored ladybeetle, Harmonia axyridis (Pallas) (Coleoptera:Coccinellidae) adults, Acta Entomologica Sinica, 2008, 51(12): 1271-1278 (in Chinese) [赵静, 于令媛, 李敏, 等. 异色瓢虫成虫耐寒能力的季节性变化. 昆虫学报, 2008, 51(12): 1271-1278]
[18]  Watanabe M. Cold tolerance and myo-inositol accumulation in overwintering adults of a lady beetle, Harmonia axyridis (Coleoptera: Coccinellidae). European Journal of Entomology, 2002, 99(1): 5-9
[19]  Koch R L, Carrillo M A, Venette R C, et al. Cold hardiness of the multicolored Asian lady beetle (Coleoptera: Coccinellidae). Environmental Entomology, 2004, 33(4): 815-822
[20]  Jing X H, Wang X H, Kang L. Chill injury in the eggs of the migratory locust, Locusta migratoria (Orthoptera: Acrididae): the time-temperature relationship with high-temperature interruption. Journal of Insect Science, 2005, 12(3): 171-178
[21]  更多...
[22]  Nedvěd O, Lavy D, Verhoef H A. Modelling the time-temperature relationship in cold injury and effect of high temperature interruptions on survival in a chill-sensitive collembolan. Functional Ecology, 1998, 12(5): 816-824
[23]  Jing Xiaohong, Kang Le. Overview and evaluation of research methodology for insect cold hardiness , Chinese Bulletin of Entomology , 2004, 41(1): 7-10 (in Chinese) [景晓红, 康乐. 昆虫耐寒性的测定与评价方法. 昆虫知识, 2004, 41(1): 7-10
[24]  Zachariassen K E, Kristiansen E, Pedersen S A. Inorganic ions in cold hardiness. Cryobiology, 2004, 48(2): 126-133
[25]  Overgaard J, S?rensen J G, Petersen S O, et al. Changes in membrane lipid composition following rapid cold hardening in Drosophila melanogaster. Journal of Insect Physiology, 2005, 51(11): 1173-1182
[26]  Rojas R R, Leopold R A. Chilling injury in the housefly: evidence for the role of oxidative stress between pupariation and emergence. Cryobiology, 1996, 33(4): 447-458
[27]  Xue Fangsen, Wei Hongyi, Zhu Xingfen. On the catalase activity in diapausing pupae of striated white butterfly. Journal of Plant Protection, 1997, 24(3): 204-208 (in Chinese) [薛芳森, 魏洪义, 朱杏芬. 黑纹粉蝶滞育蛹体内过氧化氢酶活力的研究. 植物保护学报, 1997, 24(3): 204-208]
[28]  Slachta M, Berková P, Vambera J, et al. Physiology of the cold acclimation in non-diapausing adults of Pyrrhocoris apterus (Heteroptera). European Journal of Entomology, 2002, 99(2): 181-187
[29]  Wang H S, Zhou C S, Guo W, et al. Thermoperiodic acclimations enhance cold hardiness of the eggs of the migratory locust. Cryobiology, 2006, 53(2): 206-217

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