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棉铃虫C型凝集素抑制多角体病毒体内增殖

, PP. 468-473

Keywords: 棉铃虫,C型凝集素,RNA干扰,棉铃虫核型多角体病毒

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Abstract:

为探究C型凝集素(C-typelectin)在病毒传播中的可能作用,采用半定量RT-PCR法检测了棉铃虫Helicoverpaarmigera不同C型凝集素在核型多角体病毒(nuclearpolyhedrosisvirus,NPV)感染后的表达变化,并通过real-timeqPCR和RNA干扰技术研究了NPV在棉铃虫体内的增殖状况及对棉铃虫致死率的影响。结果显示,在感染NPV后,棉铃虫血细胞C型凝集素Ha-lectin1和Ha-lectin5基因表达变化明显,其中Ha-lectin1在血细胞和脂肪体中的表达显著上调;注射dsRNA后,Ha-lectin1基因相对于actin的表达量均值由0.19下降到0.04,下降幅度达79%,显示Ha-lectin1基因被成功干扰。随着Ha-lectin1基因的沉默,病毒滴度由0.0015快速升高到1.95,棉铃虫的死亡率也随之增加到97.7%。表明C型凝集素能够抑制NPV在棉铃虫体内的增殖。

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