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枯草芽胞杆菌PTS-394的GFP标记及其定殖能力

, PP. 416-422

Keywords: 枯草芽胞杆菌PTS-394,绿色荧光蛋白,定殖

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Abstract:

为探明枯草芽胞杆菌PTS-394在番茄根围的定殖能力,采用电转化法获得绿色荧光蛋白(greenfluorescentprotein,GFP)标记菌株PTS-GFP,构建其生长曲线,采用对峙生长法评价其室内抑菌活性,并应用抗生素平板回收结合激光扫描共聚焦显微镜观察标记菌株在番茄根围的定殖数量。结果显示:与原始菌株PTS-394相比,标记菌株PTS-GFP的生长、对青枯病菌和4种病原真菌的室内抑菌能力无明显差异。标记菌株PTS-GFP和青枯病菌菌液单独或混合处理番茄苗,灌根当天标记菌株初始菌量接近108CFU/g,处理3d后种群数量迅速下降,约106CFU/g,随后缓慢下降,处理10d后种群数量约104CFU/g,处理30d后,标记菌株仍然能被检测到,约20CFU/g。表明枯草芽胞杆菌PTS-394在番茄根际土壤中具有一定的定殖能力。

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