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三种杀菌剂对牡丹黑斑病菌菌丝生长及分生孢子萌发的影响

, PP. 367-372

Keywords: 牡丹黑斑病,杀菌剂,菌丝生长,孢子萌发,致畸作用

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Abstract:

为筛选防治牡丹黑斑病的化学药剂,采用菌丝生长速率法和涂布平板法分别测定了多菌灵、苯醚甲环唑和嘧菌酯对牡丹黑斑病菌菌丝生长的抑制活性及对分生孢子萌发和形成的影响。结果表明:苯醚甲环唑对菌丝生长的抑制作用最强,有效中浓度EC50仅为0.30μg/mL,其次为嘧菌酯6.34μg/mL和多菌灵92.18μg/mL;多菌灵可显著降低分生孢子的萌发速率,而苯醚甲环唑和嘧菌酯对孢子萌发的抑制作用较弱,可使分生孢子及芽管发生畸形,并改变分生孢子的萌发方式;3种药剂均可显著抑制芽管的伸长生长,推迟病菌分生孢子梗的形成时间,但对分生孢子的产生数量无显著影响。多菌灵可作为保护剂在病害发生前期使用,苯醚甲环唑和嘧菌酯可在病害发生期轮换使用。

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