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抗精?唑禾草灵的日本看麦娘ACCase基因突变

, PP. 447-452

Keywords: 日本看麦娘,精?唑禾草灵,乙酰辅酶A羧化酶,抗药性,基因突变

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Abstract:

为明确日本看麦娘抗性种群对精?唑禾草灵的抗性水平及抗性产生的分子机制,采用整株水平测定法测定了日本看麦娘对精?唑禾草灵的抗性水平,扩增和比对了日本看麦娘抗性和敏感种群间乙酰辅酶A羧化酶(acetyl-CoAcarboxylase,ACCase)基因的差异.结果显示,与敏感种群AH-7相比,抗性种群AH-25对精?唑禾草灵的抗性倍数为33.82;AH-25种群ACCase基因CT区域2078位氨基酸发生了突变,由天冬氨酸GAT突变为甘氨酸GGT;AH-25种群对炔草酯、烯草酮和烯禾啶产生了高水平的抗性,抗性倍数分别为35.66、38.64和29.14,对高效氟吡甲禾灵产生了低水平的抗性,抗性倍数为3.04,对精喹禾灵和唑啉草酯较敏感.表明ACCase基因2078位氨基酸的突变可能是导致精?唑禾草灵产生高水平抗性的重要原因.

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