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苏云金芽胞杆菌一新菌株的鉴定及其杀虫活性

, PP. 410-417

Keywords: 菌株鉴定,微生物杀虫剂,亚洲玉米螟

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Abstract:

为分离并鉴定对亚洲玉米螟具有高毒力的苏云金芽胞杆菌菌种,通过棋盘式采集法从吉林省农业科学院试验田土壤中分离获得野生菌株,进行了形态、生化特性、伴孢晶体观察及基因型鉴定,并测定了其对3种鳞翅目害虫的室内生物活性.结果显示:该菌株在LB培养基上为圆形、暗白色菌落,边缘不整齐,革兰氏染色阳性,产生的伴孢晶体形状多为不规则形.16SrDNA序列与苏云金芽胞杆菌属的NBRC13865同源性达99%;该菌株为cry2+cry9复合基因型,编码氨基酸序列与Cry2Ab和Cry9Ea蛋白同源性分别为94%和99%,鉴定为苏云金芽胞杆菌,命名为JN001.该菌株对亚洲玉米螟具有较高毒性,以1.0×109个/mL菌体浓度接种3龄幼虫72h后,其校正死亡率为95.06%,致死中浓度(LC50)为4.12×103个/mL菌体,而对斜纹夜蛾和粘虫毒性较弱,接种72h后校正死亡率分别为12.88%和7.34%.表明该菌株是1株对亚洲玉米螟具有较强毒力的苏云金芽胞杆菌新菌株,具有较好的开发利用潜力.

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