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普通小麦-华山新麦草易位系9020-17-25-6抗条锈性遗传分析及分子标记

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Keywords: 小麦条锈病,华山新麦草,抗病基因,遗传分析

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Abstract:

为明确普通小麦-华山新麦草易位系9020-17-25-6的抗条锈病基因及其遗传特点,利用中国条锈菌小种CYR29对9020-17-25-6、铭贤169及其杂交后代F1、F2、F3代进行苗期抗条锈性鉴定及遗传分析,选取48条RGAP引物和491对SSR引物对接种CYR29的F2代群体进行筛选,寻找与抗病基因连锁的分子标记.结果表明:9020-17-25-6对CYR29具有良好的抗条锈性,由1对显性基因独立控制,暂定名为YrHua9020.筛选到2个RGAP标记(M1和M2)和位于染色体3AS上的4个SSR标记(Xwmc11、Xwmc532、Xcfd79、Xgwm2)与YrHua9020连锁,与目的基因的遗传距离分别为6.9、9.5、17.8、12.2、7.2和17.8cM.与已定位于3A染色体上的抗条锈病基因的比较研究表明,YrHua9020是一个与已知基因不同的新的抗条锈病基因.

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