全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

我国禾谷缢管蚜微卫星位点扩增稳定性及遗传多样性

, PP. 297-303

Keywords: 禾谷缢管蚜,微卫星,遗传多样性,扩增稳定性,多态性

Full-Text   Cite this paper   Add to My Lib

Abstract:

为筛选用于我国禾谷缢管蚜种群遗传学研究的微卫星位点,从8个省(市)共9个地区采集282头试虫,采用微卫星PCR产物荧光标记与自动扫描分型方法,研究了8个欧洲禾谷缢管蚜微卫星位点在试虫个体中的扩增稳定性和遗传多样性.结果显示:位点R1.35在9个种群中均不能扩增;位点R5.29b只在7个种群的少数样本中能扩增;位点R2.73、R3.171、R5.10、R5.138、R5.50和R6.3在各种群中均能稳定扩增,这6个位点的无效等位基因频率为0.0044~0.2663,平均等位基因数为2.9~9.3个,观测杂合度为0.047~0.912,其中位点R6.3具有较低的观测杂合度(0.047)和等位基因数(2.9),不适合用于种群遗传多样性研究,而位点R2.73、R3.171、R5.10、R5.138和R5.50均具有较高的杂合度和等位基因数,可用于我国禾谷缢管蚜的种群遗传学研究.

References

[1]  Balloux F, Lugon-Moulin N. 2002. The estimation of population differentiation with microsatellite markers. Molecular Ecology, 11(2): 155-165
[2]  Botstein D, White RL, Skolnick M, Davis RW. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 32(3): 314-331
[3]  Brookfield JFY. 1996. A simple new method for estimating null allele frequency from heterozygote deficiency. Molecular Ecology, 5(3): 453-455
[4]  Cao YZ, Yin J, Li KB, Zhang KC, Li XQ. 2006. The reasons of resurgence and control strategies of wheat aphids. Plant Protection, 32(5): 72-75 (in Chinese) [曹雅忠, 尹娇, 李克斌, 张克诚, 李贤庆. 2006. 小麦蚜虫不断猖獗原因及控制对策的探讨. 植物保护, 32(5): 72-
[5]  Chapuis MP, Estoup A. 2007. Microsatellite null alleles and estimation of population differentiation. Molecular Biology and Evolution, 24(3): 621-631
[6]  Chen MH, Dorn S. 2010. Microsatellites reveal genetic differentiation among populations in an insect species with high genetic variability in dispersal, the codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae). Bulletin of Entomological Research, 100(1): 75-85
[7]  Chen MH, Han ZJ. 2006. Cloning and sequence analysis of 2 different acetylcholinesterase genes in Rhopalosiphum padi and Sitobion avenae. Genome, 49(3): 239-243
[8]  Delmotte F, Leterme N, Gauthier JP, Rispe C, Simon JC. 2002. Genetic architecture of sexual and asexual populations of the aphid Rhopalosiphum padi based on allozyme and microsatellite markers. Molecular Ecology, 11(4): 711-723
[9]  Excoffier L, Lischer HEL. 2010. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources, 10(3): 564-567
[10]  Fei XD, Li C, Zhang QW, Zhao ZW. 2011. The effects of wheat planted adjacent to rape on wheat aphids population dynamic. Journal of Plant Protection, 38(4): 339-343 (in Chinese) [费晓东, 李川, 张青文, 赵章武. 2011. 油菜-小麦邻作模式对麦蚜种群动态的影响. 植物保护学报, 38(4): 339-
[11]  Feng YY, Li W, Sun HY, Deng YY, Yu HS, Chen HG. 2013. Genetic diversity of Gaeumannomyces graminis var. tritici populations in Huang-Huai winter wheat production region of China. Journal of Plant Protection, 40(6): 495-501 (in Chinese) [冯彦霞, 李伟, 孙海燕, 邓渊钰, 于汉寿, 陈怀谷. 2013. 黄淮麦区小麦全蚀病菌群体的遗传多样性分析. 植物保护学报, 40(6): 495-
[12]  Fenton B, Margaritopoulos J, Malloch GL, Foster SP. 2010. Micro-evolutionary change in relation to insecticide resistance in the peach-potato aphid, Myzus persicae. Ecological Entomology, 35 (S1): 131-146
[13]  Gilabert A, Simon JC, Mieuzet L, Halkett F, Stoeckel S, Plantegenest M, Dedryver CA. 2009. Climate and agricultural context shape reproductive mode variation in an aphid crop pest. Molecular Ecology, 18(14): 3050-3061
[14]  Llewellyn KS, Loxdale HD, Harrington R, Brookes CP, Clark SJ, Sunnucks P. 2003. Migration and genetic structure of the grain aphid (Sitobion avenae) in Britain related to climate and clonal fluctuation as revealed using microsatellites. Molecular Ecology, 12(1): 21-34
[15]  Lu YH, Yang T, Gao XW. 2009. Establishment of baseline susceptibility data to various insecticides for aphids Rhopalosiphum padi (Linnaeus) and Sitobion avenae (Fabricius). Acta Entomologica Sinica, 52(1): 52-58 (in Chinese) [鲁艳辉, 杨婷, 高希武. 2009. 禾谷缢管蚜和麦长管蚜玻璃管药膜法敏感毒力基线的建立. 昆虫学报, 52(1): 52-
[16]  Maudet C, Miller C, Bassano B, Breitenmoser-Würsten, Gauthier D, Obexer-Ruff G, Michallet J, Taberlet P, Luikart G. 2002. Microsatellite DNA and recent statistical methods in wildlife conservation management: applications in Alpine ibex [Capra ibex (ibex)]. Molecular Ecology, 11(3): 421-436
[17]  Men QL, Chen MH, Zhang YL, Feng JN. 2012. Amplifying stability and genetic diversity of microsatellite loci in codling moth Cydia pomonella (Lepidoptera: Tortricidae) populations in China. Journal of Plant Protection, 39(4): 341-346 (in Chinese) [门秋雷, 陈茂华, 张雅林, 冯纪年. 2012. 中国疫区内苹果蠹蛾微卫星位点的扩增稳定性及遗传多样性. 植物保护学报, 39(4): 341-
[18]  Ott J. 1999. Analysis of human genetic linkage. Baltimore: Johns Hopkins University Press, pp. 24-36
[19]  Paetkau D, Strobeck C. 1995. The molecular basis and evolutionary history of a microsatellite null allele in bears. Molecular Ecology, 4(4): 519-520
[20]  Papura D, Simon JC, Halkett F, Delmotte F, Le Gallic JF, Dedryver CA. 2003. Predominance of sexual reproduction in Romanian populations of the aphid Sitobion avenae inferred from phenotypic and genetic structure. Heredity, 90(5): 397-404
[21]  更多...
[22]  Park SDE. 2001. Trypanotolerance in West African cattle and the population genetic effects of selection. Ph.D Thesis. Dublin: University of Dublin
[23]  Ravel S, Hervé JP, Diarrassouba S, Kone A, Cuny G. 2002. Microsatellite markers for population genetic studies in Aedes aegypti (Diptera: Culicidae) from C?te d\'Ivoire: evidence for a microgeographic genetic differentiation of mosquitoes from Bouaké. Acta Tropica, 82(1): 39-49
[24]  Rousset F. 2008. GENEPOP\'007: a complete reimplementation of the GENEPOP software for Windows and Linux. Molecular Ecology Resources, 8(1): 103-106
[25]  Schuelke M. 2000. An economic method for the fluorescent labeling of PCR fragments. Nature Biotechnology, 18(2): 233-234
[26]  Selkoe KA, Toonen RJ. 2006. Microsatellites for ecologists: a practical guide to using and evaluating microsatellite markers. Ecology Letters, 9(5): 615-629
[27]  Simon JC, Rispe C, Sunnucks P. 2002. Ecology and evolution of sex in aphids. Trends in Ecology & Evolution, 17(1): 34-39
[28]  Vorwerk S, Forneck A. 2006. Reproductive mode of grape phylloxera (Daktulosphaira vitifoliae, Homoptera: Phylloxeridae) in Europe: molecular evidence for predominantly asexual populations and a lack of gene flow between them. Genome, 49(6): 678-688
[29]  Wilson ACC, Massonnet B, Simon JC, Prunier-Leterme N, Dolatti L, Llewellyn KS, Figueroa CC, Ramirez CC, Blackman RL, Estoup A, et al. 2004. Cross-species amplification of microsatellite loci in aphids: assessment and application. Molecular Ecology Notes, 4(1): 104-109
[30]  Yan LN, Zhang DX. 2004. Effects of sample size on various genetic diversity measures in population genetic study with microsatellite DNA markers. Acta Zoologica Sinica, 50(2): 279-290 (in Chinese) [闫路娜, 张德兴. 2004. 种群微卫星DNA分析中样本量对各种遗传多样性度量指标的影响. 动物学报, 50(2): 279-
[31]  Zhang GX, Zhong TS. 1983. Economic insect fauna of China fasc. 25 Homoptera, Aphidinea. Beijing: Science Press, pp. 356-362 (in Chinese) [张广学, 钟铁森. 1983. 中国经济昆虫志,第二十五册,同翅目蚜虫类(一). 北京: 科学出版社, pp. 356-
[32]  Zhang XC, Zhou GH, Shi M, Fang JZ, Zhao ZP, Li SH, Dong QZ, Wei K. 1985. The rules of long distance migration and virus transmission of wheat aphids. Journal of Plant Protection, 12(1): 9-16 (in Chinese) [张向才, 周广和, 史明, 方建中, 赵争平, 李淑华, 董庆周, 魏凯. 1985. 麦蚜远距离迁飞和传毒规律的研究. 植物保护学报, 12(1): 9-
[33]  Zheng Y, Peng X, Liu GM, Pan HY, Dorn S, Chen MH. 2013. High genetic diversity and structured populations of the oriental fruit moth in its range of origin. PLoS ONE, 8(11): e78476
[34]  Simon JC, Blackman RL, Le Gallic JF. 1991. Local variability in the life cycle of the bird cherry-oat aphid, Rhopalosiphum padi (Homoptera: Aphididae) in western France. Bulletin of Entomological Research, 81(3): 315-322
[35]  Simon JC, Leterme N, Delmotte F, Martin O, Estoup A. 2001. Isolation and characterization of microsatellite loci in the aphid species, Rhopalosiphum padi. Molecular Ecology Notes, 1(1/2): 4-5

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133